植物乳杆菌WW对高脂血症大鼠体脂的影响

doi:10.7506/spkx1002-6630-20180312-145

食品科学 ›› 2019, Vol. 40 ›› Issue (9): 139-145.doi: 10.7506/spkx1002-6630-20180312-145

植物乳杆菌WW对高脂血症大鼠体脂的影响

郭晶晶，乌日娜，安飞宇，李洋，祝新媛，武俊瑞*

沈阳农业大学食品学院，辽宁沈阳 110866

出版日期:2019-05-15 发布日期:2019-05-28
基金资助:
国家自然科学基金面上项目（31470538；31471713）；辽宁省“百千万人才工程”人选科技活动支持项目；沈阳市高层次科技创新人才项目（RC70212）

Effect of Lactobacillus plantarum WW on Lipid Profiles of Hyperlipidemic Rats

GUO Jingjing, WU Rina, AN Feiyu, LI Yang, ZHU Xinyuan, WU Junrui*

College of Food, Shenyang Agricultural University, Shenyang 110866, China

Online:2019-05-15 Published:2019-05-28

摘要/Abstract

摘要： 为了研究植物乳杆菌WW（Lactobacillus plantarum WW）对高脂血症大鼠体脂的影响，将32 只5 周龄雄性SD大鼠随机分为4 组：正常对照组（A）喂食基础饲料＋无菌水、高脂模型组（B）饲喂高脂饲料＋无菌水、脱脂乳对照组（C）饲喂高脂饲料＋无菌脱脂乳、菌液干预组（D）饲喂高脂饲料＋菌悬液（1×1010 CFU/mL），灌胃量为10 mL/kg mb，12 周后测定相关指标。结果显示：4 组大鼠肾脏、脾脏和心脏指数差异不显著（P＞0.05），而B组和C组肝脏指数显著高于A组和D组（P＜0.05），说明L. plantarum WW无毒副作用，并且能够减少脂肪在肝脏的堆积；B组血清和肝脏血脂水平显著高于A组（P＜0.05），说明造模成功；与B组相比，D组血清和肝脏各指标水平显著降低（P＜0.05），粪便中总胆固醇、甘油三酯和总胆汁酸含量显著升高（P＜0.05），说明L. plantarum WW能够促进胆固醇的排泄，进而降低大鼠体内胆固醇。这些结果表明植物乳杆菌WW可能是缓解高脂血症并用于功能性食品的潜在益生菌。

关键词: 植物乳杆菌WW, 高脂血症, 大鼠, 降胆固醇效果

Abstract: This research was undertaken in order to investigate the effect of Lactobacillus plantarum WW on lipid profiles of hyperlipidemic rats. A total of 32 SD male rats (5 weeks old) were randomly divided into 4 groups: normal control (A); high-fat diet model (B), skim milk control (C), and bacterial suspension intervention (D). The rats in group A were fed a normal diet plus sterile water, while those in the remaining groups were fed a high-fat diet plus sterile water, sterile skim milk and the bacterial suspension of this strain (1 × 1010 CFU/mL), respectively. The volume of intragastric administration was 10 mL/kg mb. After the 12-week experimental period, serum, tissue and fecal samples were collected for measurement of lipid profiles. The results showed that there was no significant difference in kidney, spleen or heart indexes among the four groups (P > 0.05). Liver index was significantly higher in group B and C than in group A and D (P < 0.05), indicating that L. plantarum WW was safe without toxic side effect and could reduce the accumulation of fat in the liver. Serum and liver lipid levels in group B were significantly higher than those in group A (P < 0.05), indicating that the hyperlipidemic rat model was successfully established. Compared with group B, serum and liver lipid levels in group D significantly decreased (P < 0.05), while total cholesterol (TC), triglyceride (TG) and total bile acid (TBA) in feces increased significantly (P < 0.05). Therefore, L. plantarum WW can promote the excretion of cholesterol, and consequently reduce cholesterol in rats. This strain can be used as a potential probiotic in alleviating hyperlipidemia and functional foods.

Key words: Lactobacillus plantarum WW, hyperlipidemic, rat, cholesterol-lowering effect

中图分类号:

Q93.337

郭晶晶，乌日娜，安飞宇，李洋，祝新媛，武俊瑞. 植物乳杆菌WW对高脂血症大鼠体脂的影响[J]. 食品科学, 2019, 40(9): 139-145.

GUO Jingjing, WU Rina, AN Feiyu, LI Yang, ZHU Xinyuan, WU Junrui. Effect of Lactobacillus plantarum WW on Lipid Profiles of Hyperlipidemic Rats[J]. FOOD SCIENCE, 2019, 40(9): 139-145.

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植物乳杆菌WW对高脂血症大鼠体脂的影响

Effect of Lactobacillus plantarum WW on Lipid Profiles of Hyperlipidemic Rats

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