原花青素B2对D-半乳糖模型小鼠肠道菌群的影响

doi:10.7506/spkx1002-6630-20180402-005

摘要/Abstract

摘要： 以D-半乳糖皮下注射诱导小鼠衰老模型，探究原花青素B2对衰老小鼠肠道菌群结构的影响。将C57/BL小鼠随机分为对照组、衰老组和原花青素B2组，7 周后测定其十二指肠及结肠组织中总抗氧化能力、超氧化物歧化酶活力、过氧化物酶活力和丙二醛含量，利用Illumina MiSeq高通量测序技术检测其肠道菌群16S rDNA基因，探索原花青素B2与衰老小鼠肠道菌群之间的关系，利用气相色谱-质谱联用仪检测小鼠粪便短链脂肪酸（shortchain fatty acids，SCFAs）含量。结果表明：D-半乳糖模型小鼠的十二指肠及结肠的抗氧化酶活力显著下降，MDA含量显著上升（P＜0.05）；在原花青素B2干预后，小鼠体内的多种抗氧化酶活力上升，MDA含量显著降低（P＜0.05），与对照组没有显著差异（P＞0.05）；利用高通量测序技术发现3 组小鼠肠道菌群中主要发生变化的是norank-f-Bacteroidales-s24-7、Bacteroides、Blautia、unclassified-Lachnospiraeae、Ruminiclostridium、Lachnospiraeae-NK4A136、Roseburia 7 种菌属；相比于对照组，衰老组norank-f-Bacteroidales-s24-7的相对丰度显著降低，其他菌属的相对丰度显著上升（P＜0.05）；与衰老组相比，原花青素B2组norank-f-Bacteroidales-s24-7、Roseburia、Lachnospiraeae-NK4A136和Bacteroides的相对丰度显著上升，而Blautia、unclassified-Lachnospiraeae和Ruminiclostridium的相对丰度显著降低（P＜0.05）。原花青素B2可提高衰老小鼠体内SCFAs含量，改善肠道环境，进而延缓衰老进程。

关键词: 原花青素B2, D-半乳糖, 肠道菌群, 菌群分析

Abstract: The objective of the study is to investigate the effect of procyanidins B2 on the intestinal microflora in D-galactose-induced aging mouse model. Thirty C57/BL mice were randomly and equally divided into control, aging model and procyanidins B2 treatment groups. After an experimental period of seven weeks, total antioxidant capacity, superoxide dismutase activity and catalase activity, and malondialdehyde (MDA) content as a lipid peroxidation marker were detected in the duodenum and colon. High-throughput sequencing of the 16S rDNA gene of the intestinal flora was performed using an Illumina MiSeq platform to explore the relationship between procyanidin B2 and the intestinal flora in aging mice. The contents of short-chain fatty acids (SCFAs) in mouse feces were determined by gas chromatography-mass spectrometry. The results showed that the activity of antioxidant enzymes was significantly decreased in the duodenum and colon of aging mice, and the content of MDA was significantly increased (P < 0.05). After intervention with procyanidins B2, antioxidant enzyme and MDA levels were restored to values not significantly different from normal (P > 0.05). The major differential intestinal microorganisms were norank-f-Bacteroidales-s24-7, Bacteroides, Blautia, unclassified-Lachnospiraeae, Ruminiclostridium, Lachnospiraeae-NK4A136, and Roseburia among all groups of mice. Compared with the control group, the proportion of norank-f-Bacteroidales-s24-7 in the aging group was decreased significantly, and the proportions of the other strains were increased significantly (P < 0.05). Compared with the aging group, the proportions of norank-f-Bacteroidales-s24-7, Roseburia, Lachnospiraeae-NK4A136 and Bacteroides were significantly increased in the mice treated with procyanidins B2, while the proportion of Blautia, unclassified-Lachnospiraeae, and Ruminiclostridium was significantly decreased (P < 0.05). Accordingly, procyanidins B2 could regulate the contents of SCFAs in aging mice to improve the intestinal environment, thereby delaying the aging process.

Key words: procyanidin B2, D-galactose, intestinal flora, analysis of flora

中图分类号:

TS201.4

訾雨歌，徐越，肖瀛，吴其国，尹志婷，周一鸣，周小理. 原花青素B2对D-半乳糖模型小鼠肠道菌群的影响[J]. 食品科学, 2019, 40(9): 146-151.

ZI Yuge, XU Yue, XIAO Ying, WU Qiguo, YIN Zhiting, ZHOU Yiming, ZHOU Xiaoli. Effect of Procyanidin B2 on Intestinal Microorganisms in D-Galactose-Induced Aging Mouse Model[J]. FOOD SCIENCE, 2019, 40(9): 146-151.

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原花青素B2对D-半乳糖模型小鼠肠道菌群的影响

Effect of Procyanidin B2 on Intestinal Microorganisms in D-Galactose-Induced Aging Mouse Model

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