关键词: 杜氏藻, 杜氏藻, 类胡萝卜素, 叶黄素, ?-胡萝卜素, pH值, 波长

Abstract: This study aims to explore the influences of variation in the original pH of culture media and different illumination wavelength on carotenoids, including ?-carotene and lutein, amounts from Dunaliella salina during culture. In experiments, media with different pH were prepared and used to culture Dunaliella salina for 105days. Alga culture density was then countered while ?-carotene and lutein amounts were assessed from culture liquid. Under illumination at full range, 630nm and 430nm wavelength, medium with pH=7 was applied to culture alga for 40 days. Alga culture density was then countered while ?-carotene and lutein amounts were assessed from culture liquid. Data from the experiments suggested that: (1) Dunaliella salina exhibited an ability to adjust environmental pH to become neutral. After 105-day culture, an increased alga culture density was seen from culture liquids with original pH=10-13, pH=1-3 and pH=4-9 while the maximum alga culture density was counted at pH=4. Higher ?-carotene and lutein amounts were observed from culture liquids with original medium pH=7-9 while higher ?-carotene and lutein amounts were seen from single cell with original medium pH=10-13. (2) It was observed that alga culture density could increase under illumination at the full-range wavelength, red (630nm) and blue light (430nm) after 40-day culture. Both red and blue lights were able to induce the increased amounts of ?-carotene and lutein from single cell while the blue light was the maximum.

Key words: Dunaliella salina, Dunaliella, Carotenoid, Lutein, ?-Carotene, pH, Wavelength