食品科学

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同时蒸馏萃取和固相微萃取对双孢蘑菇风味物质的提取分析

吕艳杰   

  1. 威尔德(北京)香精有限公司,北京 100176
  • 出版日期:2015-05-25 发布日期:2015-05-08

Comparative Application of SDE and SPME for Analysis of Aroma-Active Compounds in Button Mushroom

LÜ Yanjie   

  1. Wild Flavors (Beijing) Co. Ltd., Beijing 100176, China
  • Online:2015-05-25 Published:2015-05-08

摘要:

采用同时蒸馏萃取法和固相微萃取法对双孢蘑菇的挥发性风味物质进行提取,并通过气相色谱-质谱联用分析及鉴定。采用同时蒸馏萃取法鉴定出36 种化合物,采用固相微萃取法鉴定出34 种风味化合物,2 种方法分析出蘑菇的特征风味物质为1-辛烯-3-醇、1-辛烯-3-酮、2-辛烯-1-醇、2,6-二甲基吡嗪、2-戊基呋喃、甲基吡嗪、1-辛醇等,均以1-辛烯-3-醇含量最高。2 种提取方式相互补充相互验证。

关键词: 双孢蘑菇, 同时蒸馏萃取, 固相微萃取, 气相色谱-嗅闻-质谱联用

Abstract:

Aroma-active compounds in Agarucs bisporus were isolated by simultaneous distillation extraction (SDE) or solid
phase micro extraction (SPME) and analyzed by gas chromatography-olfactometry-mass spectrometry (GC-O-MS). A total
of 36 and 34 kinds of aroma-active compounds were identified by SDE and SPME, respectively. The following characteristic
flavor compounds were identified: 1-octene-3-ol, 1-octene-3-one, 2-octene-1-ol, 2,6-dimethyl pyrazine, 2-pentyl furan,
methyl pyrazine, n-octanol etc. 1-Octene-3-ol was identified by both methods as the most abundant compound. The two
extraction methods can be used to complement and validate each other.

Key words: Agaricus bisporus, simultaneous distillation extraction (SDE), solid phase micro extraction (SPME), gaschromatography-olfactometry-mass spectrometry (GC-O-MS)

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