关键词: 蛋白酶, 基因工程技术, 克隆表达

Abstract:

Although proteases are widely applied in various fields, large-scale industrial application of proteases has been
restricted by many factors. Genetic engineering technology has been used for the cloning and expression of protease genes to
obtain the protease resources with high quality, high purity and high activity. The classification, structures and functions of
proteases, and strain screening for protease production are reviewed in this article. Meanwhile, the cloning, and prokaryotic
and eukaryotic expression of protease genes are discussed. Moreover, the future development in protease gene engineering
technology is also prospected.

Key words: protease, genetic engineering technology, cloning and expression