食品科学
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樊晓博，谢兰心
出版日期:
发布日期:
基金资助:
渭南职业技术学院青年科研基金项目（WZYQ201405）
FAN Xiaobo, XIE Lanxin
Online:
Published:
摘要：
固相包被恩诺沙星抗体，辣根过氧化物酶标记的抗原与标准品（或样品）中氟喹诺酮药物竞争结合抗体，建立了高效、高灵敏的氟喹诺酮药物直接竞争酶联免疫吸附分析检测方法。优化反应条件后，得到方法的IC50为2.04 μg/L，灵敏度为0.15 μg/L，线性范围0.3～15 μg/L；方法可以检测12 种氟喹诺酮药物，在生乳、鸡肉、鱼肉和虾肉4 种样品中12 种药物的回收率为70%～121.5%。
关键词: 氟喹诺酮, 酶标抗原, 多残留检测, 直接竞争酶联免疫吸附分析
Abstract:
In this study, a rapid and high sensitive direct competitive enzyme-linked immunoassay (dc-ELISA) method based on antigen labeled by horseradish peroxidase (HRP) was established and successfully applied to detect fluoroquinolones (FQs) in animal-derived food. In the direct competitive assay, monoclonal antibody was bound to the surface of a microtiter plate, and the standards (or the sample) competed with antigen for the antibody binding sites. Under optimized assay conditions, the IC50 of dc-ELISA was 2.04 μg/L, the limit of detection was 0.15 μg/L, and the linear range was 0.3–15 μg/L. This method could detect 12 kinds of FQs with recoveries from milk, chicken, fish and shrimp in the range from 70% to 121.5%.
Key words: fluoroquinolones, horseradish peroxidase (HRP)-labeled antigen, multiresidue determination, direct competitive ELISA (dc-ELISA)
中图分类号:
TS207.3
樊晓博，谢兰心. 酶标抗原直接竞争ELISA检测食品中氟喹诺酮类药物多残留[J]. 食品科学, doi: 10.7506/spkx1002-6630-201524049.
FAN Xiaobo, XIE Lanxin. Direct Competitive ELISA with HRP-Labeled Antigen for Multiresidue Determination of Fluoroquinolones in Animal-Derived Food[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524049.
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https://www.spkx.net.cn/CN/Y2015/V36/I24/265
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