超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸

doi:10.7506/spkx1002-6630-201524052

食品科学

超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸

陈晓峰1，周围2,*，解迎双2

1.西北师范大学地理与环境科学学院，甘肃兰州 730070；2.甘肃出入境检验检疫局综合技术中心，甘肃兰州 730010

出版日期:2015-12-25 发布日期:2015-12-24
通讯作者: 周围

Direct Determination of Taurine in Infant Milk Powder by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

CHEN Xiaofeng1, ZHOU Wei2,*, XIE Yingshuang2

1. College of Geography and Environmental Science, Northwest Normal University, Lanzhou 730070, China;
2. Central Laboratory of Technical Center of Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730010, China

Online:2015-12-25 Published:2015-12-24
Contact: ZHOU Wei

摘要/Abstract

摘要：

采用超高效液相色谱串联质谱技术，以婴幼儿乳粉中的牛磺酸为研究对象，从样品处理方法，基质效应以及影响电喷雾离子源的电离因素3 个方面进行优化，建立了一个样品处理简单、快捷，基质效应低，灵敏度高的检测方法。结果表明，方法的回收率在72%～97%之间，相对标准偏差为2.16%～3.33%。该方法的仪器检出限为50 μg/L，方法检出限为1 mg/L，应用外标法定量，能够达到欧盟及我国婴幼儿乳粉中牛磺酸的检测要求。整个分析过程在8 min内完成，适合于大批量检测婴幼儿乳粉中牛磺酸的测定。

关键词: 超高效液相色谱串联质谱, 基质效应, 婴幼儿乳粉, 牛磺酸

Abstract:

Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was applied for the
direct determination of taurine in infant milk powder. The sample pretreatment procedure was optimized, and the matrix
effect and the factors influencing electrospray ionization (ESI) were evaluated to establish a simple, rapid, low-matrixeffect
and sensitive method for determining taurine. The results showed that the recovery rate of this method was between
72% and 97%, with relative standard deviation ranging from 2.16% to 3.33%. The instrument detection limit was 50 μg/L,
and the method detection limit was 1 mg/L. Quantification using an external standard method could meet the requirements
of the European Union and China for taurine detection in infant milk powder. The whole analysis can be accomplished in 8
minutes, and is suitable for the determination of taurine in mass detection of infant milk powder.

Key words: ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), matrix effects, baby milk powder, taurine

中图分类号:

TS252.7

陈晓峰，周围，解迎双. 超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸[J]. 食品科学, doi: 10.7506/spkx1002-6630-201524052.

CHEN Xiaofeng1, ZHOU Wei2,*, XIE Yingshuang2. Direct Determination of Taurine in Infant Milk Powder by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524052.

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超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸

Direct Determination of Taurine in Infant Milk Powder by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

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