响应面试验优化超临界CO2萃取人参挥发油的工艺

doi:10.7506/spkx1002-6630-201604010

食品科学

响应面试验优化超临界CO2萃取人参挥发油的工艺

崔丽丽，逄世峰，李亚丽，郑培和，赵卉，王英平

中国农业科学院特产研究所，吉林长春 130112

出版日期:2016-02-25 发布日期:2016-02-23
通讯作者: 王英平
基金资助:
吉林市科技计划项目（201452005）；中国农业科学院科技创新工程项目（CAAS-ASTIP-2014-ISAPS）

Optimization of Supercritical-CO2 Fluid Extraction of Essential oil from Panax ginseng Roots by Response Surface Methodology

CUI Lili, PANG Shifeng, LI Yali, ZHENG Peihe, ZHAO Hui, WANG Yingping

Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Online:2016-02-25 Published:2016-02-23
Contact: WANG Yingpi

摘要/Abstract

摘要：

以生晒参为原料，响应面分析法优化超临界CO2提取挥发油的工艺。采用自动质谱退卷积定性系统和保留指数方法对挥发油化学成分定性。结果表明：最佳工艺条件为萃取压力38 MPa、萃取温度55 ℃、静态萃取时间2 h、动态萃取时间1 h，此条件下挥发油提取率达1.12%。气相色谱-质谱法结果显示分离出35 个色谱峰，鉴定出29 种化学成分，相对含量占挥发性物质的96.69%。

关键词: 人参挥发油, 超临界, 响应面试验, 自动质谱退卷积定性系统, 保留指数

Abstract:

The supercritical-CO2 fluid extraction conditions of volatile oil from dried raw ginseng were optimized by
response surface methodology. Chemical constituents of the essential oil were analyzed by gas chromatography-mass
spectrometry (GC-MS) combined with automated mass spectral deconvolution and identification system (AMDIS) based
on retention index. The optimal extraction conditions were determined as follows: extraction pressure, 38 MPa; extraction
temperature, 55 ℃; static extraction time, 2 h and dynamic extraction, 1 h, under which, the maximum oil yield of 1.12%
was obtained. A total of 35 chromatographic peaks were separated from the essential oil of ginseng roots, 29 of which were
identified, accounting for 96.69% of the total peak area.

Key words: ginseng essential oil, supercritical fluid extraction, response surface methodology, automated mass spectral deconvolution and identification system (AMDIS), retention index

中图分类号:

TS224.3

崔丽丽，逄世峰，李亚丽，郑培和，赵卉，王英平. 响应面试验优化超临界CO2萃取人参挥发油的工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201604010.

CUI Lili, PANG Shifeng, LI Yali, ZHENG Peihe, ZHAO Hui, WANG Yingping. Optimization of Supercritical-CO2 Fluid Extraction of Essential oil from Panax ginseng Roots by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201604010.

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响应面试验优化超临界CO2萃取人参挥发油的工艺

Optimization of Supercritical-CO2 Fluid Extraction of Essential oil from Panax ginseng Roots by Response Surface Methodology

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