番茄红素在大鼠肝脏中的几何异构体组成

doi:10.7506/spkx1002-6630-201613034

摘要/Abstract

摘要：

目的：测定大鼠肝脏组织中番茄红素的含量及番茄红素几何异构体（顺/反异构体）的组成，为番茄红素在体内的代谢及生物学功能研究提供参考。方法：选用大鼠为动物模型，用番茄提取物配制灌胃液。一次灌胃8 h后取大鼠肝脏组织，提取其中的番茄红素。应用C30-高效液相色谱-光电二极管阵列（C30-high performance liquidchromatography-photodiode array detection，C30-HPLC-PDA）法测定提取物中番茄红素的总量和几何异构体组成。结果：大鼠肝脏中总番茄红素含量为92.95 μg/g（以鲜质量计）。与灌胃液中的番茄红素几何异构体组成比较，大鼠肝脏组织中存在更多数量和种类的顺式异构体，包括13Z-番茄红素、11Z-番茄红素、9Z-番茄红素和5Z-番茄红素异构体。结论：这一现象对研究番茄红素在肝脏内的贮存、靶向调动和生物学功能具有重要意义。

关键词: 番茄红素, 几何异构体, 肝脏, 代谢

Abstract:

Total lycopene amount and lycopene geometric isomer composition of rat liver were determined in this study,
aiming to lay a foundation for in vivo studies on lycopene metabolism and biological function. In the experiment, male
rats were administrated with tomato extract at a single dose. After 8 h, liver tissues were collected for the extraction of
lycopene. The total amount and geometric isomer composition of lycopene were determined by C30-high performance liquid
chromatography with photodiode array detection (C30-HPLC-PDA). Results indicated that total amount of lycopene from
liver tissues was 92.95 μg/g fresh weight. In comparison with tomato extract, the composition and amount of lycopene
Z-isomers (including 13Z-, 11Z-, 9Z- and 5Z-isomer) were increased in rat liver tissues. This phenomenon is important for
investigating the storage, targeting transfer and biological function of lycopene in liver.

Key words: lycopene, geometric isomer, liver, metabolism

中图分类号:

TS201.2

惠伯棣，冯润月，宫平. 番茄红素在大鼠肝脏中的几何异构体组成[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613034.

HUI Bodi, FENG Runyue, GONG Ping. Geometric Isomer Composition of Lycopene from Rat Liver[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613034.

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