黑果枸杞中一种花色苷类物质的分离纯化及抗氧化活性的研究

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黑果枸杞中一种花色苷类物质的分离纯化及抗氧化活性的研究

唐骥龙¹,闫亚美²,曹有龙²,曾晓雄¹,孙怡¹,胡冰¹,周莉¹

1. 南京农业大学
2. 国家枸杞工程技术研究中心

收稿日期:2015-10-01 修回日期:2016-06-21 出版日期:2016-12-25 发布日期:2016-08-30
通讯作者: 闫亚美 E-mail:yanyamei@163.com
基金资助:
宁夏自然科学基金;宁夏农林科学院先导项目;江苏高校优势学科建设工程资助项目

Purification and antioxidant activity of one anthocyanin in Lycium ruthenicum Murray

Received:2015-10-01 Revised:2016-06-21 Online:2016-12-25 Published:2016-08-30
Contact: yamei yan E-mail:yanyamei@163.com

摘要/Abstract

摘要： 以黑果枸杞为原料，利用AKTA Purifier蛋白纯化系统分离纯化(色谱条件：YMC-PACK ODS-A色谱柱，流动相为体积分数4%甲醇/19%乙腈/1%甲酸溶液，等度洗脱，流速2 mL/min)得到1种纯度97%以上的花色苷物质。通过质谱和核磁共振分析，鉴定该花色苷物质为矮牵牛素3-O-芸香糖（反式-香豆酰基）-5-O-葡萄糖苷（petunidin 3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-[β-D-glucopyranoside]）。体外抗氧化活性分析表明该花色苷物质具有显著的DPPH自由基和超氧阴离子自由基清除能力。

关键词: 黑果枸杞, 花色苷, 纯化, 结构鉴定, 抗氧化活性

Abstract: One kind of anthocyanin was purified from Lycium ruthenicum Murray by AKTA purifier protein purification system (Chromatographic conditions: YMC-PACK ODS-A column and methanol（4%）/acetonitrile(19%)/formic acid (1%) solution as mobile phase at a flow rate of 2 ml/min). The purified anthocyanin was identified as petunidin 3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-[β-D-glucopyranoside] by mass spectrometry and nuclear magnetic resonance spectrometry. Furthermore, the purified anthocyanin showed potent scavenging activities on 1,1-diphenyl-2-picrylhydrazyl free radical and superoxide anion in terms of antioxidant assays in vitro.

Key words: Lycium ruthenicum Murray, anthocyanin, purification, structural identification, antioxidant activity

中图分类号:

Q946.83 TS201.2

唐骥龙闫亚美曹有龙曾晓雄孙怡胡冰周莉. 黑果枸杞中一种花色苷类物质的分离纯化及抗氧化活性的研究[J]. 食品科学, 0, (): 0-0.

参考文献

[1] ZHENG Jie, DING Chenxu, WANG Liangsheng, et al. Anthocyanins composition and antioxidant activity of wild Lycium ruthenicum Murr. from Qinghai-Tibet Plateau[J]. Food Chemistry, 2011, 126(3): 859-865.
[2] STINTZING F C, CARLE R. Functional properties of anthocyanins and betalains in plants food and in human nutrition[J]. Trends in Food Science & Technology, 2004, 15(1): 19-38
[3] 李进, 原惠, 曾献春, 等. 黑果枸杞色素的毒理学研究[J]. 食品科学, 2007, 28(7): 470-475.
[4] KONG J M, CHIA L S, GOH N K, et al. Analysis and biological activities of anthocyanins[J]. Phytochemistry, 2003, 64(5): 923-933.
[5] 闫亚美, 戴国礼, 冉林武, 等. 不同产地野生黑果枸杞资源果实多酚组成分析[J]. 中国农业科学, 2014, 47(22): 4540-4550.
[6] 闫亚美, 代彦满, 冉林武, 等. 黑果枸5种果蔬中花色苷组成及体外抗氧化活性比较[J]. 食品工业科技, 2014, 35(16): 133-136.
[7] 林丽, 李进, 丁成丽. 高效液相色谱法测定黑果枸杞果实中花色苷的含量[J]. 食品科学, 2013, 34(6): 164-166.
[8] CASTANEDA-OVANDO A, DE LOURDES PACHECO-HERNáNDEZ M, PáEZ-HERNáNDEZ M E, et al. Chemical studies of anthocyanins: A review[J]. Food Chemistry, 2009, 113(4): 859-871.
[9] 李进, 瞿伟菁, 吕海英, 等. 黑果枸杞色素的提取和精制工艺研究 [J]. 天然产物研究与开发, 2006, 18(4): 650-654.
[10] 李淑珍, 李进, 杨志江, 等. 大孔树脂分离纯化黑果枸杞总黄酮的研究[J]. 食品科学, 2009 ,30(1):19-24
[11] JIN Hongli, LIU Yanfang, GUO Zhimou, et al. High-performance liquid chromatography separation of cis–trans anthocyanin isomers from wild Lycium ruthenicum Murr. Employing a mixed-mode reversed-phase/strong anion-exchange stationary phase[J]. Journal of Agricultural and Food Chemistry, 2015, 63(2): 500-508.
[12] JIN Hongli, LIU Yangfang, YANG Fan, et al. Characterization of anthocyanins in wild Lycium ruthenicum Murray by HPLC-DAD/QTOF-MS/MS[J]. Analytical Methods, 2015.7(39): 4947-4956
[13] 王晴川, 张鑫, 张文芹, 等. 苦丁冬青苦丁茶中咖啡酰奎尼酸类物质的分离纯化和高效液相色谱法分析[J]. 食品科学, 2013, 34(22): 119-122.
[14] HU Bing, WANG Yan, XIE Minhao, et al. Polymer nanoparticles composed with gallic acid grafted chitosan and bioactive peptides combined antioxidant, anticancer activities and improved delivery property for labile polyphenols[J]. Journal of Functional Foods, 2015, 15: 593-603.
[15] BEAUCHAMP C, FRIDOVICH I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry, 1971, 44(1): 276-287.
[16] HU Na, ZHENG Jie, LI Wencong, et al. Isolation, stability, and antioxidant activity of anthocyanins from Lycium ruthenicum Murray and Nitraria Tangutorum Bobr of Qinghai-Tibetan Plateau[J]. Separation Science and Technology, 2014, 49(18): 2897-2906.
[17] CAO Shaoqian, PAN Siyi, YAO Xiaolin, et al. Isolation and purification of anthocyanins from blood oranges by column chromatography[J]. Agricultural Sciences in China, 2010, 9(2): 207-215.
[18] CAPITANI D, SOBOLEV A P, DELFINI M, et al. NMR methodologies in the analysis of blueberries[J]. Electrophoresis, 2014, 35(11): 1615-1626.
[19] ANDO T, SAITO N, TATSUZAWA F, et al. Floral anthocyanins in wild taxa of Petunia (Solanaceae)[J]. Biochemical Systematics and Ecology, 1999, 27(6): 623-650.
[20] DOWNEY M O, ROCHFORT S. Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin[J]. Journal of Chromatography A, 2008, 1201(1): 43-47.
[21] SIMIRGIOTIS M J, SCHMEDA-HIRSCHMANN G. Direct identification of phenolic constituents in Boldo Folium (Peumus boldus Mol.) infusions by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry[J]. Journal of Chromatography A, 2010, 1217(4): 443-449.
[22] GEORGE F, FIGUEIREDO P, TOKI K, et al. Influence of trans-cis isomerisation of coumaric acid substituents on colour variance and stabilisation in anthocyanins[J]. Phytochemistry, 2001, 57(5): 791-795.

黑果枸杞中一种花色苷类物质的分离纯化及抗氧化活性的研究

Purification and antioxidant activity of one anthocyanin in Lycium ruthenicum Murray

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