食品科学 ›› 2012, Vol. 33 ›› Issue (2): 143-148.doi: 10.7506/spkx1002-6630-201202031

• 工艺技术 • 上一篇    下一篇

水酶法提取榛子蛋白工艺优化

李 杨,江连洲,王胜男,李丹丹,王 梅,刘 琪,齐宝坤   

  1. 1.东北农业大学食品学院 2.国家大豆工程技术研究中心
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    黑龙江省攻关项目(GA09B401-6);国家现代农业产业技术体系建设项目(nycytx-004)

Process Optimization for Aqueous Enzymatic Extraction of Filbert Protein

LI Yang,JIANG Lian-zhou,WANG Sheng-nan,LI Dan-dan,WANG Mei,LIU Qi,QI Bao-kun   

  1. 1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. The National Research Center of Soybean Engineering and Technology, Harbin 150030, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 以榛子仁为原料,利用Alcalase碱性蛋白酶水解,进而提取榛子蛋白。以榛子仁总蛋白提取率为指标,对影响因素进行研究。在单因素试验的基础上采用响应面法优化工艺条件,得到最佳酶解条件:加酶量2.0%、温度55℃、酶解时间2.5h、料水比1:5(g/mL)、pH8.9。由F检验可得因素贡献率为x1>x2>x4>x5>x3,即加酶量>酶解温度>料液比>酶解pH值>酶解时间。

关键词: 水酶法, 碱性蛋白酶, 榛子蛋白, 响应面

Abstract: Alcalase was used for the aqueous enzymatic extraction of protein from filbert kernel. Based on one-factor-at-a-time experiments, response surface methodology was used to optimize process conditions for maximizing total protein extraction efficiency. The optimal extraction conditions were determined as follows: enzyme dosage 2.0%, hydrolysis temperature 55 ℃, hydrolysis time 2.5 h, material/liquid ratio 1:5, and hydrolysis pH of 8.9. The results of F tests showed that the process conditions could be ranked in decreasing order of contribution rate as follows: enzyme amount, hydrolysis temperature, material/liquid ratio, pH, and hydrolysis time.

Key words: aqueous enzymatic extraction, alcalase, filbert protein, response surface methodology

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