食品科学 ›› 2012, Vol. 33 ›› Issue (5): 98-101.doi: 10.7506/spkx1002-6630-201205022

• 基础研究 • 上一篇    下一篇

源于不同加工条件的大豆分离蛋白的电子顺磁共振分析

黄友如,陈义勇,朱东兴,赵 阳,王叹玉   

  1. 常熟理工学院生物与食品工程学院
  • 出版日期:2012-03-15 发布日期:2012-03-03
  • 基金资助:
    江苏省高校自然科学研究项目(10KJB550001);苏州市科技计划项目(SYND201003)

Electron Paramagnetic Resonance Spectroscopy (EPR) Study of Soybean Protein Isolates Prepared by Different Processing Methods

HUANG You-ru,CHEN Yi-yong,ZHU Dong-xing,ZHAO Yang,WANG Tan-yu   

  1. School of Biological Science and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2012-03-15 Published:2012-03-03

摘要: 分析比较几种不同来源的大豆分离蛋白的电子顺磁共振波谱(EPR)。结果表明:制备工艺条件不同,大豆分离蛋白EPR波谱中场碳自由基信号存在差异。降低蛋白提取过程中豆粕浸提的温度或减少原料脱脂豆粕的残留脂质含量、钝化原料脱脂豆粕中脂肪氧合酶活力,可使大豆分离蛋白中场碳自由基信号显著减弱。

关键词: 大豆分离蛋白, 电子顺磁共振, 自由基

Abstract: Soybean protein isolates were prepared by different methods and comparatively analyzed by electron paramagnetic resonance (EPR) spectroscopy. The results showed that soybean protein isolates obtained by different methods were different in central singlet signal attributed to the carbon radical (g value ranged between 2.0051 and 2.0054). The central free radical signal of soybean protein isolates could be weakened by decreasing extraction temperature, reducing lipid contents of defatted soybean flakes or inactivating lipoxygenase activity.

Key words: soybean protein isolates, electron paramagnetic resonance(EPR), free radicals

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