关键词: 郫县豆瓣, 真菌演替, 高通量测序

Abstract: The changes in fungal communities in Pixian soybean paste during post-fermentation were analyzed using high-throughput sequencing method in order to reveal the essence of ‘being kept under sunlight in the daytime and being allowed to absorb moisture in the air at night during the fermentation process’. The results revealed that at the taxonomic levels of microbes, including phylum, class, order, family, and genus, high-throughput sequencing method could detect 3 phyla, 20 classes, 47 orders, 77 families and 106 genera. It was shown that the fungal community in Pixian soybean paste was highly diverse and abundant. The dominant microbes were greatly changed during the post-fermentation process; the quantities of Pleosporaceae and Sclerotiniaceae continued to reduce, while those of Saccharomycetaceae, Saccharomycodaceae and Pichiaceae increased firstly and then decreased. In addition, a large number of non-cultivated fungi and unclassified fungi were also found in this study. The observed fungal diversity was highly similar to the microbial ecology of the investigated samples. This study may provide scientific support for the modernization of the traditional Pixian soybean paste industry as well as food safety and quality control.

Key words: Pixian soybean paste, fungal community, high-throughput sequencing