液体发酵竹红菌素的稳定性及抗肿瘤活性

doi:10.7506/spkx1002-6630-201123018

食品科学 ›› 2011, Vol. 32 ›› Issue (23): 96-100.doi: 10.7506/spkx1002-6630-201123018

液体发酵竹红菌素的稳定性及抗肿瘤活性

邵颖1，陈安徽1，贺菊萍1，胡飞2，樊美珍2

1.徐州工程学院食品（生物）工程学院 2.安徽省微生物防治重点实验室

出版日期:2011-12-15 发布日期:2011-12-21

Stability and Anti-tumor Activity of Hypocrellin from Liquid-Cultured Mycelia of Shiraia bambusicola Strain ZH-5-1

SHAO Ying1， CHEN An-hui1，HE Ju-ping1， HU Fei2， FAN Mei-zhen2

(1. College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China； 2. Anhui Provincial Key Laboratory of Microbial Pest Control, Hefei 230036, China)

Online:2011-12-15 Published:2011-12-21

摘要/Abstract

摘要： 研究竹黄无性型菌株ZH-5-1液体发酵菌丝体中竹红菌素的稳定性及抗肿瘤活性。采用分光光度法测定竹红菌素溶液的吸光度以考察竹红菌素稳定性；以中国仓鼠卵巢肿瘤细胞(CHO细胞)株、人子宫颈癌细胞HeLa、HL-60人白血病细胞株和A-549人肺腺癌细胞株为模型，用Resazurin法检测液体发酵竹红菌素的抗肿瘤活性。结果显示：液体发酵竹红菌素在酸性条件下表现稳定；对热、光、H2O2、亚硫酸钠的稳定性良好；低浓度的金属离子不会影响竹红菌素稳定性，在所有供试离子中Mn2+、Cu2+、Fe2+对竹红菌素稳定性的破坏作用较大。液体发酵竹红菌素样品对4种供试肿瘤细胞株均具有生长抑制活性，对CHO、HL-60、A-549肿瘤细胞的抑制作用表现出良好的剂量-效应关系，其IC50分别为0.47、0.11、0.22mg/mL。总之，液体发酵竹红菌素是一种稳定的且具有抗肿瘤活性的天然产物，具有广阔的应用空间。

关键词: 液体发酵竹红菌素, 稳定性, 抗肿瘤活性

Abstract: The stability of hypocrellin from the liquid-cultured mycelia of a strain of anamorph (ZH-5-1) from Shiraia bambusicola was evaluated based on changes in the spectrophotometric absorbance of hypocrellin solutions. The anti-tumor activity was tested by Resazurin assay using CHO, HeLa, HL-60 and A-549 cells. The results showed that the hypocrellin was stable in acidic condition and had good thermal and light stability. Commonly used oxidant H2O2 and reducing agent Na2SO3 had no obvious effect on hypocrellin stability. The stability of the hypocrellin could not be influenced by low concentrations of metal ions, but among all the tested metal ions, Mn2+, Cu2+ and Fe2+ had more destructive effect. The hypocrellin inhibited the growth of all the four tested tumor cells. Hypocrellin inhibition on the growth of CHO, HL-60, and A-549 cells was in dose-dependent manner with an IC50 of 0.47, 0.11 mg/mL and 0.22 mg/mL, respectively. In conclusion, mycelium hypocrellin of strain ZH-5-1 is a stable natural product with anti-tumor activity and thus will have potentially promising applications in practice.

Key words: hypocrellin, stability, anti-tumor activity

中图分类号:

TS201

邵颖，陈安徽，贺菊萍，胡飞，樊美珍. 液体发酵竹红菌素的稳定性及抗肿瘤活性[J]. 食品科学, 2011, 32(23): 96-100.

SHAOYing，CHENAn-hui，HEJu-ping，HUFei，FANMei-zhen. Stability and Anti-tumor Activity of Hypocrellin from Liquid-Cultured Mycelia of Shiraia bambusicola Strain ZH-5-1[J]. FOOD SCIENCE, 2011, 32(23): 96-100.

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液体发酵竹红菌素的稳定性及抗肿瘤活性

Stability and Anti-tumor Activity of Hypocrellin from Liquid-Cultured Mycelia of Shiraia bambusicola Strain ZH-5-1

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