食品科学 ›› 2011, Vol. 32 ›› Issue (23): 215-219.doi: 10.7506/spkx1002-6630-201123043

• 生物工程 • 上一篇    下一篇

植物乳杆菌胞外多糖发酵条件的优化

冯美琴1,2,邢家溧1,张 琦1,李程程1,陈晓红1,李 伟1,董明盛1,*   

  1. 1.南京农业大学食品科技学院 2.金陵科技学院动物科学与技术学院
  • 出版日期:2011-12-15 发布日期:2011-12-21
  • 基金资助:
    国家“863”计划项目(2011AA100903);江苏省自然科学基金项目(BK2011651); 教育部高等学校博士学科点专项科研基金项目(新教师类)(20110097120028)

Optimization of Fermentation Conditions for Production of Exopolysaccharides by Lactobacillus plantarum 70810

FENG Mei-qin1,2,XING Jia-li1,ZHANG Qi1,LI Cheng-cheng1,CHEN Xiao-hong1,LI Wei1,DONG Ming-sheng1,*   

  1. (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China)
  • Online:2011-12-15 Published:2011-12-21

摘要: 目的:对植物乳杆菌胞外多糖的发酵条件进行优化。方法:采用Plackett-Burman法从影响植物乳杆菌胞外多糖(EPS)产量的14个因素中筛选出3个主要影响因素,然后通过响应面法探讨最优工艺参数。结果:影响植物乳杆菌胞外多糖产量的主要因素是蔗糖质量浓度、接种量以及发酵温度,其最佳条件为蔗糖质量浓度34g/L、接种量5%、发酵温度31℃,在此条件下发酵的植物乳杆菌胞外多糖产量达425.16mg/L。结论:应用Plackett-Burman设计和响应面法进行植物乳杆菌胞外多糖发酵条件的优化是可行的。

关键词: 植物乳杆菌, 胞外多糖, Plackett-Burman设计, 响应面法

Abstract: Objective: The fermentation conditions for producing exopolysaccharides (EPS) by Lactobacillus plantarum 70810 were optimized. Methods: Plackett-Burman (PB) design was carried out to screen three major factors out of 14 ones that affect the production of EPS from Lactobacillus plantarum 70810. Further, the screened factors were optimized using response surface methodology (RSM). Results: The production of EPS was significantly affected by sucrose concentration, inoculation amount and fermentation temperature. The optimal fermentation conditions were identified as sucrose concentration of 34 g/L, inoculation amount of 5% and fermentation temperature of 31 ℃, resulting in an EPS yield of 425.16 mg/L. Conclusion: It is feasible to apply PB design and RSM to optimize the fermentation conditions for the production of exopolysaccharides by Lactobacillus plantarum 70810.

Key words: Lactobacillus plantarum, exopolysaccharides (EPS), Plackett-Burman design, response surface methodology (RSM)

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