食品科学 ›› 2012, Vol. 33 ›› Issue (9): 77-80.doi: 10.7506/spkx1002-6630-201209017

• 基础研究 • 上一篇    下一篇

北京洋槐蜜化学成分及其抗氧化活性

孙丽萍1,穆雪峰1,2,施海燕3,徐 响1,庞 杰2   

  1. 1.中国农业科学院蜜蜂研究所 2.福建农林大学食品科学学院 3.北京百花蜂业科技发展股份公司
  • 出版日期:2012-05-15 发布日期:2012-05-07
  • 基金资助:
    “十二五”国家科技支撑计划项目(2011BAD33B04);国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18); 科技部农业科技成果转化资金项目(2009GB23260456)

Chemical Components and Antioxidant Activity of Acacia Honey from Beijing

SUN Li-ping1,MU Xue-feng1,2,SHI Hai-yan3,XU Xiang1,PANG Jie2   

  1. (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Baihua Bee Industrial Technology and Development Co. Ltd., Beijing 100176, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 采用层析法富集北京洋槐蜜中抗氧化成分,利用高效液相测定其化学组成并研究其中已知化学成分对DPPH自由基的清除作用,旨在评价北京洋槐蜜的抗氧化功能。结果显示:北京洋槐蜜中含有绿原酸、咖啡酸、对香豆酸、山奈酚、异鼠李素、松属素、白杨素和高良姜素8种已知成分,含量在9.5~257.8μg/100g之间,除松属素和白杨素外,其余各成分均有清除DPPH自由基的能力,且差异显著(P<0.01)。从有效成含量及抗氧化能力综合考虑,山奈酚可视为北京洋槐蜜中最有代表性的抗氧化成分。

关键词: 洋槐蜜, 化学成分, 抗氧化活性, DPPH自由基, 山奈酚

Abstract: Antioxidant compounds from Acacia honey from Beijing were enriched by column chromatography and detected by high-performance liquid chromatography. The antioxidant activity of these compounds was evaluated by DPPH radical-scavenging assay. Eight compounds were detected in Acacia honey, which were chlorogenic acid, caffeic acid, p-coumaric acid, kaempferol, isorhamnetin, pinocembrin, chrysin and galangin at the content range of 9.5-257.8μg/100 g. Meanwhile, a significant difference in DPPH radical-scavenging capacity was also observed. In terms of both the content and antioxidant capacity, kaempferol could be recognized as the most representative antioxidant component of Acacia honey from Beijing.

Key words: Acacia honey, chemical components, antioxidant activity, DPPH radical, kaempferol

中图分类号: