柱前衍生- 气相色谱法同时测定不同柑橘汁中的糖和肌醇

doi:10.7506/spkx1002-6630-201210036

食品科学 ›› 2012, Vol. 33 ›› Issue (10): 173-178.doi: 10.7506/spkx1002-6630-201210036

柱前衍生- 气相色谱法同时测定不同柑橘汁中的糖和肌醇

张耀海1,2，赵其阳1，张雪莲3，王磊1，焦必宁1,3,*，周志钦2

1.西南大学柑桔研究所 2.西南大学园艺园林学院 3.西南大学食品科学学院

出版日期:2012-05-25 发布日期:2012-05-07
基金资助:
国家现代农业(柑桔)产业技术体系建设专项(CARS-27)；重庆市自然科学基金项目(CSTC 2009BB1136)；第四十七批中国博士后科学基金面上项目(20100470808)；中央高校基本科研业务费项目(XDJK2012C059)

Simultaneous Determination of Inositols and Carbohydrates in Different Citrus Juices by Gas Chromatography with Pre-column Derivatization

ZHANG Yao-hai1,2，ZHAO Qi-yang1，ZHANG Xue-lian3，WANG Lei1，JIAO Bi-ning1,3,*，ZHOU Zhi-qin2

(1. Institute of Citrus Research, Southwest University, Chongqing 400712, China； 2. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China； 3. College of Food Science, Southwest University, Chongqing 400716, China)

Online:2012-05-25 Published:2012-05-07

摘要/Abstract

摘要： 为建立柱前衍生-气相色谱技术同时测定不同柑橘汁(宽皮柑橘、甜橙、柚子、柠檬和金柑)中3种肌醇(肌肌醇、鲨肌醇、手性肌醇)和3种可溶性糖(果糖、葡萄糖、蔗糖)的方法，以内标物(甲基-α-D-葡萄糖苷)定量，标准物质的保留时间定性。结果表明：在两个添加水平下，6种成分的平均回收率为98.1%～106.9%，相对标准偏差为0.6%～6.1%，检测限在0.29×10-3～0.41×10-3μg/L(RSN＝3)。本方法简便快速，结果准确可靠。

关键词: 气相色谱, 肌醇, 柑橘汁, 内标物

Abstract: A gas chromatographic method using pre-column derivatization was described for the quantitative analysis of fructose, glucose, sucrose, chiro-, scyllo- and myo-inositol in different citrus juices. Juices from different fresh citrus (loose-skin mandarin, sweet orange, pummelo, lemon and kumquat) were prepared in the laboratory. Inositols and carbohydrates were analyzed by GC as their oximes derivatives and their identities were confirmed by retention of pure standards. The method was evaluated for precision and recovery using methyl-α-D-glucoside as an internal standard. The recoveries of the method evaluated at two spiked levels were in the range of 98.1%－106.9% with RSDs from 0.6%－6.1%. The limits of detection (LODs) were from 0.29×10-3－0.41×10-3 μg/L (RSN＝3). The results support the suitability of the method. The method is simple, quick and reproducible, and applicable to confirm inositols and carbohydrates in different kinds of citrus juice.

Key words: gas chromatography (GC), inositol, citrus juice, internal standard

中图分类号:

TS255.1

张耀海，赵其阳，张雪莲，王磊，焦必宁，周志钦. 柱前衍生- 气相色谱法同时测定不同柑橘汁中的糖和肌醇[J]. 食品科学, 2012, 33(10): 173-178.

ZHANGYao-hai，ZHAOQi-yang，ZHANGXue-lian，WANGLei，JIAOBi-ning，ZHOUZhi-qin. Simultaneous Determination of Inositols and Carbohydrates in Different Citrus Juices by Gas Chromatography with Pre-column Derivatization[J]. FOOD SCIENCE, 2012, 33(10): 173-178.

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柱前衍生- 气相色谱法同时测定不同柑橘汁中的糖和肌醇

Simultaneous Determination of Inositols and Carbohydrates in Different Citrus Juices by Gas Chromatography with Pre-column Derivatization

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