关键词: 副溶血性弧菌, 裂解性噬菌体, 生物学特性, 杀菌作用, 生三文鱼

Abstract: The biological properties of a lytic phage VpJYP2 that infects Vibrio parahaemolyticus were determined, and its bactericidal effect on raw salmon fillets was analyzed as well. The results showed that the plaques of VpJYP2 were clear and sized about 1?2 mm diameter with halos around them. VpJYP2 had an icosahedral head of about 64 nm diameter and a tail of about 70 nm length which had a tail sheath of 20 nm width. VpJYP2 was identified to belong to family Myoviridae. VpJYP2 had a genome of 25 363 bp which contains 45 open reading frames, 13 of them were predicted to express certain functional proteins. VpJYP2 was stable at 40?50 ℃ and stable under pH 4?11. The optimal multiplicity of infection of VpJYP2 was 0.01 and one-step growth experiment showed that the latent time and burst time were 5 min and 55 min, respectively, and burst size was 45. VpJYP2 showed significant reduction effects on V. parahaemolyticus in raw salmon fillets. The results indicated that VpJYP2 can serve as a promising biocontrol agent against V. parahaemolyticus.

Key words: Vibrio parahaemolyticus, lytic phage, biological properties, bactericidal effect, raw salmon