食品科学 ›› 2021, Vol. 42 ›› Issue (8): 264-269.doi: 10.7506/spkx1002-6630-20200318-276

• 安全检测 • 上一篇    下一篇

上海进口水产品中副溶血性弧菌耐药性、毒力基因和遗传特征

申进玲,赵丽娜,韩伟,许镇坚,蒋原,杨捷琳,郭德华,薛峰   

  1. (1.上海海关动植物与食品检验检疫技术中心,上海 200135;2.江苏省肉类生产与加工质量安全控制协同创新中心,江苏 南京 210095)
  • 出版日期:2021-04-25 发布日期:2021-05-14
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFC1603603);上海市自然科学基金项目(19ZR1417500)

Antimicrobial Resistance, Virulence Gene and Genetic Characteristics of Vibrio parahaemolyticus Isolates from Aquatic Products Imported to Shanghai

SHEN Jinling, ZHAO Lina, HAN Wei, XU Zhenjian, JIANG Yuan, YANG Jielin, GUO Dehua, XUE Feng   

  1. (1. Technology Center for Animal Plant and Food Inspection and Qurantine, Shanghai Customs, Shanghai 200135, China; 2. Jiangsu Collaborative Innovation Center of Meat Production and Processing, Nanjing 210095, China)
  • Online:2021-04-25 Published:2021-05-14

摘要: 为研究上海进口水产品中副溶血性弧菌的耐药性、关键毒力基因携带情况以及遗传进化关系,为其科学预警和溯源提供数据支持,针对2017—2019年从上海口岸进口水产品中分离的68?株副溶血性弧菌,分析其抗生素敏感性、关键毒力基因携带情况和多位点序列分型。副溶血性弧菌对氨苄西林耐药率最高(98.53%),对其余抗生素耐药率相对较低,对左氧氟沙星和氯霉素均敏感。对9?种抗生素中介耐药,其中氨基糖苷类、哌拉西林、环丙沙星和头孢西丁中介耐药率较高。20.59%菌株耐2?种及以上抗生素。发现1?株携带trh,均不携带tdh。共鉴定出65?种ST型,其中52?种为新发现ST型。未发现菌株地域来源、食品类型与ST型之间存在明显关联,但在不同时间同一国家同种类型食品中发现相同独特ST型菌株。上海地区进口水产品中副溶血性弧菌遗传高度多样,存在中介耐药和多重耐药现象,存在高致病潜力菌株,需要引起重视。

关键词: 副溶血性弧菌;多位点序列分析;毒力基因;耐药性

Abstract: This work aimed to study the antimicrobial resistance, key virulence genes and genetic evolution of Vibrio parahaemolyticus isolated from aquatic products imported to Shanghai for the purpose of providing supportive data for the early warning of V. parahaemolyticus contamination risks and tracking its sources. A total of 68 isolates were obtained from aquatic products imported to Shanghai in the years between 2017 and 2019 and analyzed for antimicrobial susceptibility, key virulence genes and multi-locus sequence typing. These isolates showed the highest resistance rate to ampicillin (98.53%), whereas their resistance rates to other antimicrobials tested were relatively lower, and all the isolates were sensitive to levofloxacin and chloramphenicol. Intermediate resistance was observed for nine antimicrobials, among which, the intermediate resistance rates to aminoglycosides, piperacillin, ciprofloxacin and cefoxitin were relatively higher. In total, 20.59% of the isolates were resistant to two or more antimicrobials. One isolate was found to harbor trh, and no isolate harbored tdh. A total of 65 sequence types (STs) were identified, of which, 52 were novel STs. No apparent correlation was found between geographical origin, food type and ST type; however, isolates of the same specific STs were identified in the same type of food samples imported from the same country at different times. In conclusion, the V. parahaemolyticus isolates were highly genetically diverse and showed intermediate resistance and multiple resistance to drugs, and some of them were found to have high pathogenic potential. Thus, much attention should be paid to this issue.

Key words: Vibrio parahaemolyticus; multi-locus sequence typing analysis; virulence gene; antimicrobial resistance

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