NO处理对马铃薯采后块茎愈伤的影响及机制

摘要/Abstract

摘要： 【目的】研究NO处理对马铃薯块茎愈伤的影响，探讨部分机理。【方法】以“陇薯7号”马铃薯块茎为试材，人工模拟损伤后，用0.5 mM NO供体硝普钠（Sodium nitroprusside, SNP）浸泡损伤块茎10min，于常温黑暗条件下进行愈伤。测定愈伤期间损伤块茎的失重率和Fusarium sulphureum损伤接种块茎的病情指数，观察愈伤期间损伤块茎伤口处软木脂和木质素的积累，分析伤口处组织苯丙烷代谢和过氧化物酶活性以及H2O2含量。【结果】SNP处理有效降低了愈伤期间损伤块茎的失重率和损伤接种块茎的病情指数，愈伤第5 d，处理块茎的失重率和病情指数分别低于对照33.8%和31.4%。SNP处理显著促进了块茎伤口处聚酚软木脂、聚酯软木脂和木质素的积累，愈伤第5 d，处理块茎的聚酚软木脂、聚酯软木脂和木质化细胞层厚度分别高出对照25.2%、27.3%和23.6%。SNP处理还显著提高了块茎伤口处组织苯丙氨酸解氨酶活性，增加了总酚、类黄酮和木质素的积累，愈伤第14d，处理块茎分别高出对照42.9%、23.7%、28.4%和24.7%。此外，处理还显著增加了块茎伤口处的过氧化物酶活性和H2O2含量。【结论】SNP激活了马铃薯块茎伤口处组织的苯丙烷代谢，提高了过氧化物酶活性和H2O2含量，加速了伤口处软木脂和木质素的积累，促进了马铃薯块茎的采后愈伤。

关键词: 马铃薯块茎, NO, 愈伤, 苯丙烷代谢

Abstract: 【Objective】To study the effect of nitric oxide treatment on wound healing of harvested potato tubers, and to explore partial mechanisms. 【Method】The cultivar ‘Longshu No.7’ was used as material in this study, after artificially wounded, the tubers were dipped with sodium nitroprusside (SNP) of exogenous donor of NO at 0.5 mM for 10 minutes, then the treated tubers were healed at ambient temperature in darkness. The weight loss of wounded tubers and the disease index of tubers inoculated with Fusarium sulphureum were measured during healing, the accumulation of suberin and lignin was observed at wounded sites. The phenylpropanoid metabolism and peroxidase activities and H2O2 content in tissues at wounded sites were also analyzed. 【Results】The weight loss of wounded tubers and disease index of inoculated tubers were significantly reduced by SNP treatment during healing, which were 33.8% and 31.4% lower than that of control after 5 days of healing. The treatment accelerated the accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at wounded site of tubers, the thickness of cell layers of that were 25.2%, 27.3% and 23.6% higher than the control after 5 days of healing respectively. The treatments increased phenylalanine ammonia-lyase activity, and the content of total phenols, flavonoids and lignin at wounded sites, which were 42.9%, 23.7%, 28.4% and 24.7% higher than that of control after 14 days of healing respectively. In addition, SNP also increased significantly peroxidase activity and hydrogen peroxide content at wounded sites.【Conclusion】SNP treatment activated phenylpropane metabolism of potato tubers, improved peroxidase activity and H2O2 content, accelerated suberin and lignin accumulation at wounded sites. Therefore, SNP promoted the wound healing of potato tubers.

Key words: potato tubers, nitric oxide, wound healing, phenylpropane metabolism

中图分类号:

韩占红毕阳. NO处理对马铃薯采后块茎愈伤的影响及机制[J]. 食品科学, 0, (): 0-0.

Zhan-hong. Postharvest Nitric Oxide Treatment Promotes Wound Healing of Potato Tubers[J]. FOOD SCIENCE, 0, (): 0-0.

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