二氧化氯通过激活苯丙烷代谢加速马铃薯块茎的愈伤过程

doi:10.7506/spkx1002-6630-20220119-181

食品科学 ›› 2022, Vol. 43 ›› Issue (23): 211-219.doi: 10.7506/spkx1002-6630-20220119-181

二氧化氯通过激活苯丙烷代谢加速马铃薯块茎的愈伤过程

柴秀伟，孔蕊，郑晓渊，朱亚同，梁伟，赵诗佳，李宝军，毕阳，Dov PRUSKY

（1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.农业研究组织新鲜农产品采后科学部，以色列里雄莱锡安 7505101）

出版日期:2022-12-15 发布日期:2022-12-28
基金资助:
国家自然科学基金面上项目（31772040）；甘肃省马铃薯产业技术体系项目（GARS-MLS-2）

Chlorine Dioxide Accelerates the Wound Healing Process of Potato Tubers by Eliciting Phenylpropanoid Metabolism

CHAI Xiuwei, KONG Rui, ZHENG Xiaoyuan, ZHU Yatong, LIANG Wei, ZHAO Shijia, LI Baojun, BI Yang, Dov PRUSKY

(1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel)

Online:2022-12-15 Published:2022-12-28

摘要/Abstract

摘要： 二氧化氯（ClO2）是一种安全高效的消毒剂，广泛用于果蔬保鲜和疾病的防治，但ClO2处理对马铃薯采后块茎的愈伤效果尚不明确。本研究采用25 mg/L ClO2浸泡切半‘大西洋’马铃薯块茎10 min，然后于室温黑暗条件下愈伤0、3、5、7、14、21 d。测定损伤块茎在愈伤期间的质量损失率以及损伤接种硫色镰刀菌（Fusarium sulphureum）块茎的病情指数，观察伤口处聚酚软木脂和木质素的积累，测定伤口处苯丙烷代谢关键酶和过氧化物酶活力，以及苯丙烷代谢产物和H2O2的含量。结果表明，25 mg/L ClO2处理加速了聚酚软木酯和木质素的沉积，增加了愈伤期间损伤组织的细胞层厚度。此外，ClO2处理降低了损伤块茎的质量损失率以及损伤接种硫色镰刀菌（F. sulphureum）后块茎的病情指数，第14天时，处理块茎的质量损失率和病情指数分别低于对照20.8%和45.3%。另外，ClO2处理通过提高块茎伤口组织处苯丙氨酸解氨酶、肉桂酸-4-羟化酶与肉桂醇脱氢酶的活力，增加了5 种酚酸（肉桂酸、p-香豆酸、咖啡酸、阿魏酸和芥子酸）及3 种木质素单体（p-香豆醇、芥子醇和松柏醇）的含量；ClO2处理还显著促进了总酚、类黄酮和木质素的合成，提高了H2O2含量和过氧化物酶（peroxidase，POD）活力。综上所述，ClO2处理加速了愈伤期间马铃薯损伤块茎伤口处聚酚软木酯、木质素的沉积，降低了损伤块茎的质量损失率及病情指数，激活了苯丙烷代谢，提高了H2O2含量和POD活力。这些发现为ClO2处理加速马铃薯块茎的愈伤提供了理论依据。

关键词: 二氧化氯；马铃薯块茎；苯丙烷代谢；愈伤；聚酚软木酯；木质素

Abstract: Chlorine dioxide (ClO2) is a safe and effective disinfectant that is widely used for preserving fruits and vegetables as well as controlling microorganisms. However, the mechanism by which ClO2 treatment affects wound healing in potato tubers remains unclear. The half cut potato tubers ‘cv. Atlantic’ were soaked with 25 mg/L ClO2 for 10 min, which were stored for 0, 3, 5, 7, 14, and 21 d at room temperature in dark for wound healing. The mass loss rate and the disease index of wounded tubers inoculated with Fusarium sulphureum were determined, the deposition of suberin poly phenolic (SPP) and lignin at wound sites were observed, and the activities of the key enzymes of phenylpropanoid metabolism and peroxidase (POD) as well as the content of phenylpropanoid metabolism products and H2O2 at wound sites were measured. This result showed that ClO2 at 25 mg/L accelerated SPP and lignin deposition and increased cell layer thickness at wounds. Furthermore, the mass loss rate and disease index (after inoculated with F. sulphureum) of potato tubers significantly was reduced by 20.8% and 45.3%, respectively, on day 14 after ClO2 treatment. Additionally, ClO2 enhanced the contents of five phenolic acids (cinnamic, p-coumaric, caffeic, ferulic, and sinapic acids) and three lignin monomers (p-coumaryl, sinapyl, and coniferyl alcohols) by increasing phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), and cinnamyl alcohol dehydrogenase (CAD) activities. Moreover, ClO2 treatment significantly promoted the synthesis of total phenolics, flavonoids and lignin and elevated H2O2 content and peroxidase activity. In conclusion, ClO2 treatment accelerated the deposition of SPP and lignin at wound sites, decreased the mass loss rate and disease index of damaged potato tubers during healing, triggered the phenylpropanoid metabolism pathway, and increased H2O2 content and POD activity. These findings provide evidence that ClO2 treatment can accelerate wound healing in potato tubers.

Key words: chlorine dioxide; potato tubers; phenylpropanoid metabolism; wound healing; suberin poly phenolic; lignin

中图分类号:

TS255.36

柴秀伟，孔蕊，郑晓渊，朱亚同，梁伟，赵诗佳，李宝军，毕阳，Dov PRUSKY. 二氧化氯通过激活苯丙烷代谢加速马铃薯块茎的愈伤过程[J]. 食品科学, 2022, 43(23): 211-219.

CHAI Xiuwei, KONG Rui, ZHENG Xiaoyuan, ZHU Yatong, LIANG Wei, ZHAO Shijia, LI Baojun, BI Yang, Dov PRUSKY. Chlorine Dioxide Accelerates the Wound Healing Process of Potato Tubers by Eliciting Phenylpropanoid Metabolism[J]. FOOD SCIENCE, 2022, 43(23): 211-219.

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二氧化氯通过激活苯丙烷代谢加速马铃薯块茎的愈伤过程

Chlorine Dioxide Accelerates the Wound Healing Process of Potato Tubers by Eliciting Phenylpropanoid Metabolism

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