羧甲基壳聚糖诱导培养罗伦隐球酵母处理调控采后葡萄柚果实苯丙烷代谢及其抗青霉病作用

doi:10.7506/spkx1002-6630-20220630-339

食品科学 ›› 2023, Vol. 44 ›› Issue (11): 160-166.doi: 10.7506/spkx1002-6630-20220630-339

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羧甲基壳聚糖诱导培养罗伦隐球酵母处理调控采后葡萄柚果实苯丙烷代谢及其抗青霉病作用

田登娟，孔珊珊，马电通，李根前，王芳，邓佳

（1.西南林业大学林学院，云南昆明 650224；2.红河学院生物科学与农学学院，云南红河 661199；3.西南地区生物多样性保育国家林业和草原局重点实验室，云南昆明 650224；4.西南山地森林资源保育与利用教育部重点实验室，云南昆明 650224）

发布日期:2023-06-30
基金资助:
国家自然科学基金地区科学基金项目（31960326；32160394）；云南省高层次人才培养支持计划项目（YNWR-QNBJ-2020-205）；云南省中青年学术和技术带头人后备人才及省技术创新人才培养对象项目（202105AC160045）；云南省农业联合专项（202101BD070001-065）

Regulation of Phenylpropane Metabolism and Resistance to Green Mold in Postharvest Grapefruits Treated by Carboxymethyl Chitosan-Induced Culture of Cryptococcus laurentii

TIAN Dengjuan, KONG Shanshan, MA Diantong, LI Genqian, WANG Fang, DENG Jia

(1. College of Forestry, Southwest Forestry University, Kunming 650224, China; 2. College of Biological and Agricultural Sciences, Honghe University, Honghe 661199, China; 3. Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China; 4. Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Kunming 650224, China)

Published:2023-06-30

摘要/Abstract

摘要： 为研究羧甲基壳聚糖（carboxymethyl chitosan，CMCS）诱导培养罗伦隐球酵母（Cryptococcus laurentii）处理对采后葡萄柚果实青霉病害的防控作用，以‘里约红’葡萄柚为试材，蒸馏水为对照，研究CMCS、C. laurentii单独处理和CMCS诱导培养C. laurentii（记为CMCS-C. laurentii）浸泡处理对葡萄柚果实青霉病害的防控效果及其对果实苯丙烷代谢的影响。结果表明，CMCS-C. laurentii处理显著抑制了葡萄柚果实青霉病发病率及病斑直径的扩展（P＜0.05）。此外，与对照组相比，CMCS-C. laurentii处理提高了葡萄柚果实苯丙氨酸解氨酶、肉桂酸-4-羟基化酶、4-香豆酸辅酶A连接酶、多酚氧化酶、过氧化物酶、β-1,3-葡聚糖酶和几丁质酶活力以及总酚、类黄酮、木质素的含量。综上，添加CMSC诱导培养提高了C. laurentii的生防效力，CMCS-C. laurentii处理通过提高采后葡萄柚果实的苯丙烷代谢能力和抗性物质含量，从而增强葡萄柚果实抗青霉病效力，且CMCS-C. laurentii处理果实抗病效果优于CMCS、C. laurentii单独处理。

关键词: 葡萄柚；羧甲基壳聚糖；罗伦隐球酵母；苯丙烷代谢；诱导抗病

Abstract: The purpose of this study was to investigate the effect of carboxymethyl chitosan (CMCS)-induced culture of Cryptococcus laurentii treatment on the resistance to Penicilium italicum in postharvest grapefruits. ‘Rio Red’ grapefruits were dipped in distilled water as a control, CMCS solution, C. laurentii suspension or CMCS-induced C. laurentii suspension. The biocontrol efficacy against green mold and phenylpropane metabolism in the treated grapefruits were analyzed. The results showed that the CMCS-induced C. laurentii treatment significantly reduced the disease incidence and lesion expansion of P. italicum in grapefruits (P < 0.05). CMCS-induced C. laurentii treatment induced an increase in the activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumaric acid coenzyme A ligase (4CL), polyphenol oxidase (PPO), peroxidase (POD), β-1,3-glucanase (GLU), and chitinase (CHI), and the contents of total phenols, flavonoids and lignin compared to the control group. Therefore, CMCS can induce increased biocontrol efficacy of C. laurentii. CMCS-induced C. laurentii can enhance the resistance to Penicilium italicum in postharvest grapefruits by increasing phenolpropane metabolism and resistance substance contents, and its effect is more pronounced than those of CMCS and C. laurentii.

Key words: Citrus paradisi; carboxymethyl chitosan; Cryptococcus laurentii; phenolpropane metabolism; induced disease resistance

中图分类号:

TS255.36

田登娟，孔珊珊，马电通，李根前，王芳，邓佳. 羧甲基壳聚糖诱导培养罗伦隐球酵母处理调控采后葡萄柚果实苯丙烷代谢及其抗青霉病作用[J]. 食品科学, 2023, 44(11): 160-166.

TIAN Dengjuan, KONG Shanshan, MA Diantong, LI Genqian, WANG Fang, DENG Jia. Regulation of Phenylpropane Metabolism and Resistance to Green Mold in Postharvest Grapefruits Treated by Carboxymethyl Chitosan-Induced Culture of Cryptococcus laurentii[J]. FOOD SCIENCE, 2023, 44(11): 160-166.

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羧甲基壳聚糖诱导培养罗伦隐球酵母处理调控采后葡萄柚果实苯丙烷代谢及其抗青霉病作用

Regulation of Phenylpropane Metabolism and Resistance to Green Mold in Postharvest Grapefruits Treated by Carboxymethyl Chitosan-Induced Culture of Cryptococcus laurentii

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