食品科学 ›› 2010, Vol. 31 ›› Issue (23): 243-247.doi: 10.7506/spkx1002-6630-201023054

• 生物工程 • 上一篇    下一篇

Neurospora.crassa降解茶粕培养基粗纤维的发酵工艺研究

肖玉娟,邓泽元* ,范亚苇,李 静,刘 蓉,胡蒋宁   

  1. 南昌大学 食品科学与技术国家重点实验室,高等研究院
  • 收稿日期:2010-07-26 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 邓泽元 E-mail:dengzy28@yahoo.com.cn
  • 基金资助:

    2010 年江西省科技重大专项;食品科学与技术国家重点实验室目标导向课题(SKLF-MB-201003)

Fermentation Processing of Fiber Degradation from Oil-tea-cake Residue by Neurospora. crassa

XIAO Yu-juan,DENG Ze-yuan*,FAN Ya-wei,LI Jing,LIU Rong,HU Jiang-ning   

  1. (State Key Laboratory of Food Science and Technology, Institute for Advanced Study, Nanchang University,
    Nanchang 330047, China)
  • Received:2010-07-26 Online:2010-12-15 Published:2010-12-29
  • Contact: DENG Ze-yuan E-mail:dengzy28@yahoo.com.cn

摘要:

以提取茶皂素后的茶粕为材料,研究Neurospora.crassa(粗壮脉纹胞菌)降解茶粕培养基粗纤维的发酵条件。在单因素试验的基础上,选定豆渣、米糠、含水量及发酵时间进行四因素三水平Box-Behnken 实验,建立粗纤维降解率的二次回归方程,通过响应面分析得到优化组合工艺条件。结果表明:最佳的茶粕发酵条件为豆渣添加量29.38%、米糠添加量18.54%、含水量72%、发酵时间74.9h 时,培养基粗纤维降解率达到最大值。该条件下粗纤维降解率预测值为48.65%,验证值为48.36%。

关键词: 粗壮脉纹胞菌, 茶粕, 发酵条件, 响应曲面法优化

Abstract:

The fermentation processing for fiber degradation from the residue of oil-tea-cake after the extraction of tea saponin was investigated. According to results of single-factor experiments, soybean residue, rice bran, water content and fermentation time were selected for the Box-Behnken design to establish a quadric regression equation for describing the degradation of fiber. The optimal fermentation conditions were achieved by response surface analysis to be soybean residue of 29.38%, rice bran of 18.54%, water content of 72% and fermentation time of 74.9 h. Under the optimal fermentation conditions, the highest degradation of fiber reached up to 48.65%, which was consistent with 48.36% of the validated value.

Key words: Neurospora. crassa, oil-tea-cake, fermentation processing, response surface methodology

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