关键词: 固相萃取, 流动注射化学发光, 沙拉沙星, 鸡蛋

Abstract:

A high sensitive and fast method was developed for determination of sarafloxacin(SAR) in eggs. Sarafloxacin in sample was extracted with phosphate, and purified by solid phase extraction (SPE). Since sarafloxacin could significantly enhanced the reaction between luminol and potassium ferricyanide, it could be detected by flow injection-chemiluminescence method. The linear ranges of this method was 6×10-9－6×10-5 mol/L, and the detection limit was 3.97×10-11 mol/L. The average recovery was 96.2%, and the relative stand deviation (RSD) was 1.71%(n=11).

Key words: solid phase extraction, flow injection-chemiluminescence, sarafloxacin, eggs