暴马丁香抑菌活性、抗氧化作用研究及微量元素的测定

doi:10.7506/spkx1002-6300-201007029

食品科学 ›› 2010, Vol. 31 ›› Issue (7): 131-134.doi: 10.7506/spkx1002-6300-201007029

暴马丁香抑菌活性、抗氧化作用研究及微量元素的测定

张崇禧¹，蔡恩博²，陈艳²，李金平²，黄坤²，关键²，薛长春²，郑友兰^{2 ,*}

1.山东大学威海分校海洋学院 2.吉林农业大学中药材学院

收稿日期:2009-05-14 出版日期:2010-04-01 发布日期:2010-12-29
通讯作者: 郑友兰 E-mail:sqcg126621@126.com

Ethanol Extract of Syringa reticulate: Evaluation of Antibacterial Activity and Antioxidant Activity and Analysis of Trace Elements

ZHANG Chong-xi1，CAI En-bo2，CHEN Yan2，LI Jin-ping2，HUANG Kun2，GUAN Jian2，XUE Chang-chun2，ZHENG You-lan2,*

1. Marine College, Shandong University at Weihai, Weihai 264209, China；
2. College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China

Received:2009-05-14 Online:2010-04-01 Published:2010-12-29
Contact: ZHENG You-lan E-mail:sqcg126621@126.com

摘要/Abstract

摘要：

目的：对暴马丁香的抑菌活性、抗氧化作用进行研究，并测定其微量元素的含量。方法：以暴马香茎皮80% 乙醇提取物为材料，采用滤纸片法对5 种常见细菌的生长抑制活性、最小抑菌浓度(MIC)进行测定，并讨论温度、pH 值及紫外线对提取物抑菌效果的影响。采用DPPH 法测定其抗氧化作用；采用原子吸收分光光度法测定其微量元素Fe、Mn、Zn、Cu 的含量。结果：对金黄色葡萄球菌的MIC 为1.563mg/mL，对沙门氏菌和巴氏菌的MIC 均为6.25mg/mL。高温、酸性环境等对抑菌作用均无明显影响。其提取物50% 抑制浓度(IC50)为0.042mg/mL。微量元素含量Fe 为880.5μg/g、Mn 为26.03μg/g、Zn 为60.9μg/g、Cu 为10.13μg/g。结论：暴马丁香茎皮有着良好的抑菌效果及较强的抗氧化作用，其微量元素含量较高，具有很好的开发价值。

关键词: 暴马丁香, 抑菌, 抗氧化, 微量元素

Abstract:

Objectives: To evaluate the antibacterial activities of 80% ethanol extract of Syringa reticulate stem bark against 5 common bacteria and its antioxidant activity and analyze trace elements. Methods: The minimal inhibitory concentrations (MICs) of Syringa reticulata extract against bacteria tested were determined by filter paper method. The effects of temperature, pressure, pH and ultraviolet irradiation on antibacterial activity of the extract were also investigated. DPPH free radical scavenging assay was used for antioxidant activity evaluation. The contents of trace elements such as iron, manganese, zinc and copper were determined using atomic absorption spectrophotometry. Results: The MICs of the extract was 1.563 mg/mL against Staphylococcus aureus, and 6.25 mg/mL against both Pasteurella and Salmonella enterica. The extract exhibited stable antibacterial activity when exposed to high temperature, ultraviolet light and acidic environment. The EC50 (median effective concentration) of the extract for scavenging DPPH free radicals was 0.042 mg/mL. The contents of trace elements such as Fe, Mn, Zn and Cu were 880.5, 26.03, 60.9μg/g and 10.13 μg/g, respectively. Conclusion: Syringa reticulata stem bark has an excellent antibacterial activity and antioxidant activity and exhibits high levels of trace elements. This demonstrates its promising development potential.

Key words: Syringa reticulata, antibacterial activity, antioxidant activity, trace elements

中图分类号:

张崇禧1，蔡恩博2，陈艳2，李金平2，黄坤2，关键2，薛长春2，郑友兰2,*. 暴马丁香抑菌活性、抗氧化作用研究及微量元素的测定[J]. 食品科学, 2010, 31(7): 131-134.

ZHANG Chong-xi1，CAI En-bo2，CHEN Yan2，LI Jin-ping2，HUANG Kun2，GUAN Jian2，XUE Chang-chun2，ZHENG You-lan2,*. Ethanol Extract of Syringa reticulate: Evaluation of Antibacterial Activity and Antioxidant Activity and Analysis of Trace Elements[J]. FOOD SCIENCE, 2010, 31(7): 131-134.

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暴马丁香抑菌活性、抗氧化作用研究及微量元素的测定

Ethanol Extract of Syringa reticulate: Evaluation of Antibacterial Activity and Antioxidant Activity and Analysis of Trace Elements

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