Isolation,Purification and Anti-complement Activity of Water-soluble Polysaccharides from Chaenomeles cathayensis

Abstract

Abstract: The crude water-soluble polysaccharides from Chaenomeles cathayensis fruits were isolated with hot water extraction followed by ethanol precipitation.The polysaccharides were deproteinized using Sevag method,decolorized by macroporous resin and then dialyzed.The fine water-soluble Chaenomeles cathayensis polysaccharides(CCP) were obtained and further fraction-ated to elute for the component CCP1 by anion exchange column chromatography on DEAE-cellulose.The purity of CCP1 was examined by gel filtration chromatography of Sepharose Cl-6B.The purified polysaccharide CCP1 was proved to be homoge-neous by HPGPC and its molecular weight(MW) was 2.879×106 D.Analysis of monosaccharide composition by HPLC indicated that CCP1 consists of rhamnose,arabinose,fructose,mannitose,glucose and their molar ratio is 0.034:0.228:0.045:0.055:0.638.The main fraction CCP1 was subjected to the complement-fixation experiment.CCP1 expressed some anti-complementary activity in vitro and dosage-effect relationship between them existing.

Key words: Chaenomeles cathayensis, polysaccharide, isolation, purification, anti-complement activity

WANG Wen-Ping, GUO Si-Yuan, LI Lin, SUN Qian-Yun, TANG Wei-Yuan. Isolation,Purification and Anti-complement Activity of Water-soluble Polysaccharides from Chaenomeles cathayensis[J]. FOOD SCIENCE, 2008, 29(5): 120-124.

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Isolation,Purification and Anti-complement Activity of Water-soluble Polysaccharides from Chaenomeles cathayensis

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