Fingerprinting of Volatile Composition of Rape Honey

Abstract

Abstract: In order to label the botanical and geographical origin of unioral rape honey and discriminate adulterated rape honey, the volatile components in 49 rape honey samples from different geographical origins were analyzed by ultrasonic-assisted extraction, gas chromatography with flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) and fingerprinted. The results indicated that 49 samples investigated could be assigned into 5 groups, with an effective discrimination between raw and commercial rape honeys. Both rape honeys exhibited an obvious difference in their volatile components. We deduce that benzoic acid-4-hydroxy-3,5-dimethoxy-hydrazide has the potential to be used as a marker of botanical origin of rape honey.

Key words: rape honey, ultrasonic-assisted solvent extraction (USE), gas chromatography-mass spectrometry (GC-MS), fingerprint

CLC Number:

S896.1

References

[1] THE COUNCIL OF THE EUROPEAN UNION. 2001/110/EC-2001, Council directives [S]. Brussels: Official Journal of the European Communities, 2001.
[2] 徐风. 我国蜂产品质量安全监管水平显著提升[N]. 中国质量报, 2010-8-4(001).
[3] 赵静. 与时俱进开展我国蜂产品可追溯体系研究[J]. 中国蜂业, 2010, 61(5): 19.
[4] AMPUERO S, BOGDANOV S, BOSSET J. Classification of unifloral honeys with an MS-based electronic nose using different sampling modes: SHS, SPME and INDEX[J]. Eur Food Res Technol, 2004, 218: 198–207.
[5] KA?KONIENè V, VENSKUTONIS P R, ?EKSTERYTè V. Composition of volatile compounds of honey of various ?oral origin and beebread collected in Lithuania[J]. Food Chemistry, 2008, 111: 988-997.
[6] SORIR A C, GONZáLEZ M, SANZ J, et al. Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopalynological, physicochemical and volatile composition data[J]. Food Chemistry, 2004, 85: 121-130.
[7] ALIFERIS K A, TARANTILIS P A , HARIZANIS P C, et al. Botanical discrimination and classi?cation of honey samples applying gas chromatography/mass spectrometry ?ngerprinting of headspace volatile compounds[J]. Food Chemistry, 2010, 121: 856-862.
[8] FUENTE E, VALENCIA-BARRERA R, MARTíNEZ-CASTRO I, et al. Occurrence of 2-hydroxy-5-methyl-3-hexanone and 3-hydroxy-5-methyl-2-hexanone as indicators of botanic origin in eucalyptus honeys[J]. Food Chemistry, 2007, 103: 1176-1180.
[9] BERTELLI D, PAPOTTI G, LOLLI M, et al. Development of an HS-SPME-GC method to determine the methyl anthranilate in Citrus honeys[J]. Food Chemistry, 2008, 108: 297-303.
[10] ALISSANDRAKIS E, TARANTILIS P A,HARIZANIS P C, et al. Aroma investigation of uni?oral Greek citrus honey using solid-phase microextraction coupled to gas chromatographic–mass spectrometric analysis[J]. Food Chemistry, 2007, 100: 396-404.
[11] PONTES M, MARQUES J C, C?MARA J S. Screening of volatile composition from Portuguese multi?oral honeys using headspace solid-phase microextraction-gas chromatography–quadrupole mass spectrometry[J]. Talanta, 2007, 74: 91-103.
[12] 李成斌, 林瑜, 邓国宾等. 八种蜂蜜挥发性成分分析[J]. 精细化工, 2006, 23(11): 1082-1088.
[13] 孙丽阳. 蜂蜜挥发性成分的SPME-GC/MS分析[J]. 鞍山师范学院学报, 2007, 9(6)：33-35.
[14] 谢建春. 现代香味分析技术及应用[M]. 北京: 中国标准出版社, 2008: 69.
[15] ALISSANDRAKISA E, DAFERERAB D, TARANTILISB P A, et al. Ultrasound-assisted extraction of volatile compounds from citrus ?owers and citrus honey[J]. Food Chemistry, 2003,82:575-582.
[16] JERKOVI? I, MASTELI? J, MARIJANOVI? Z. A variety of volatile compounds as markers in unifloral honey from Dalmatian Sage[J]. Chemistry & biodiversity, 2006, 3:1307-1315.
[17] JERKOVI? I, TUBEROSO C, MARIJANOVI? Z. Headspace, volatile and semi-volatile patterns of Paliurus spina-christi uni?oral honey as markers of botanical origin[J]. Food Chemistry , 2009, 112: 239-245.
[18] JERKOVI? I, MASTELI? J, MARIJANOVI? Z. Comparison of hydrodistillation and ultrasonic solvent extraction for the isolation of volatile compounds from two uni?oral honeys of Robinia pseudoacacia L and Castanea sativa L[J]. Ultrasonics Sonochemistry, 2007, 14: 750-756.
[19] RIAL-OTERO R, GASPAR E M, MOURA I, et al. Gas chromatography mass spectrometry determination of acaricides from honey after a new fast ultrasonic-based solid phase micro-extraction sample treatment[J]. Talanta , 2007, 71:1906-1914.
[20] ALISSANDRAKIS E, TARANTILIS P A, HARIZANIS P C. Evaluation of four isolation techniques for honey aroma compounds[J]. Journal of the Science of Food and Agriculture, 2005, 85: 91-97
[21] 国家药品监督管理局. 中药注射剂指纹图谱研究的技术要求(暂行)[J]. 中成药, 2000, 22(10): 671-675.
[22] CUEVAS-GLORY L F, PINO J A, SANTIAGO L S, et al. A review of volatile analytical methods for determining the botanical origin of honey[J]. Food Chemistry, 2007, 103: 1032-1043.
[23] RADOVIC B S, CARERI M, MANGIA A, et al. Contribution of dynamic headspace GC-MS analysis of aroma compounds to authenticity testing of honey[J]. Food Chemistry, 2001, 72: 511-520.