FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (1): 171-174.

Previous Articles     Next Articles

Analysis of Antibiotics Related Genes from Antagonistic Bacillus amyloliquefaciens against Aeromonas hydrophila

Hai-Peng Cao   

  • Received:2011-10-12 Revised:2012-12-13 Online:2013-01-15 Published:2013-01-07
  • Contact: Hai-Peng Cao

Abstract: The antibiotics related genes of antagonistic Bacillus amyloliquefaciens G1 against Aeromonas hydrophila isolated from sturgeons were amplified by PCR and sequenced. Meanwhile, the amino acid sequence, transmembrane heices, domains and secondary structure of their encoded products were determined. The results showed that only the gene essential for iturin synthetase was present in strain G1, which was naturally clustered with the iturin gene family from Bacillus sp. in GenBank, including iturin A, bacillomycin D, mycosubtilin, and showed 98% similarity to the iturin A gene sequences of B. subtilis strain MH25 and strain RB14. The amino acid sequence of the encoded product of the iturin synthetase gene exhibited high similarity to that of iturin, iturin A, bacillorin and bacillomycin D, and was close relative to the iturin A synthetase B of B. subtilis strain MH25 (GenBank accession No.ABY89499). In addition, no obvious transmembrane structure was present in the encoded product of the iturin synthetase gene of strain G1. However, both AMP-binding site and PP-binding site were present in its domains. Alpha helix, beta turn, random coil and extended strand were also present in its secondary structure.

Key words: Bacillus amyloliquefaciens, antibiotics related gene, iturin synthetase essential gene, phylogenetic analysis

CLC Number: