Effect of mtnN Gene on Biosynthesis of S-Adenosylmethionine in Bacillus amyloliquefaciens

doi:10.7506/spkx1002-6630-20190124-316

Abstract

Abstract: In order to explore the effect of mtnN on S-adenosylmethionine (SAM) synthesis in Bacillus amyloliquefaciens, a series of genetically engineered strains with modifications in the mtnN gene were constructed by gene knockout and antisense RNA suppression technology in this study. Compared with the original strain HZ-12, the SAM yield and biomass of the mtnN-deleted strain decreased by 51% and 26%, respectively, indicating that complete blocking of the mtnN gene is not conducive to cell growth and SAM synthesis. Furthermore, the expression of mtnN was inhibited by antisense RNA fine-tuning. Compared with the control strain HZ-12/pHY300, the biomass of the engineered strains HZ-12/pHY-mtnNasRNA-1, HZ-12/pHY-mtnNasRNA-2 and HZ-12/pHY-mtnNasRNA-3 showed no significant difference from each other, and their SAM yields reached 14.58, 12.27 and 12.49 mg/L, respectively, increasing by 44%, 22% and 24% compared with the control, respectively. This study not only explains the influence of mtnN gene on SAM synthesis, but also provides a new strategy for construction of high-yield SAM producing engineered strains.

Key words: S-adenosylmethionine, Bacillus amyloliquefaciens, S-adenosylhomocysteine nucleosidase, antisense RNA, metabolic engineering

CLC Number:

TS201.3

RUAN Liying, WEN Zhiyou, WEI Xuetuan. Effect of mtnN Gene on Biosynthesis of S-Adenosylmethionine in Bacillus amyloliquefaciens[J]. FOOD SCIENCE, 2020, 41(6): 193-200.

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Effect of mtnN Gene on Biosynthesis of S-Adenosylmethionine in Bacillus amyloliquefaciens

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