Optimization of Derivatization Conditions for Gas Chromatographic Determination of Sec-Butylamine by Response Surface Methodology

Abstract

Abstract: Derivatization reaction is very important for the determination of sec-butylamine. For the purpose of establishing a gas chromatography-electron capture detection (GC-ECD) method for determining sec-butylamine, the optimal derivatization reaction conditions were studied by response surface methodology. Based on the results of one-factor-at-a-time experiments, a central composite design was used to investigate the pairwise interaction effects of reaction time, temperature and derivatization reagent (acetonitrile containing 0.05μL/mL pentafluorobenzaldehyde) amount on sec-butylamine recovery. The optimal derivative temperature, derivative duration and derivatization reagent amount were found to be 22.42 ℃, 31.72 min and 1.25 mL, respectively.

Key words: sec-butylamine, residue, derivatization, response surface methodology, gas chromatography

CLC Number:

TS207.3

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