Modification of Horseradish Peroxidase Sensor by AuRu Nanoparticles

Abstract

Abstract: AuRu nanoparticles were synthesized by a reverse microemulsion method. The structure, composition, and morphology of AuRu were characterized by X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS) and high-resolution transmission electron microscopy (HRTEM). A novel biosensor was prepared by using AuRu nanoparticles to modify horseradish peroxidase (HRP). The performance of the developed sensor for determining hydrogen peroxide (H2O2) were evaluated by cyclic voltammetry (CV) and chronoamperometry. The results showed that HRP/AuRu/GC had high sensitivity and stability of determination for H2O2. The ccurrent revealed an excellent linear relationship with the negative logarithm of concentration in the range of 1 × 10-7－1× 10-3 mol/L with a linear coefficient of 0.9959. The detection limit for H2O2 was 5.02 × 10-8 mol/L. The current of electrode response still retained 91.54% of the original level after 30 days of storage.

Key words: nanoparticles, AuRu alloy, hydrogen peroxide, electrode, sensor

CLC Number:

TS207.3

References

[1] K. Kriz, M. Anderlund, D. Kriz. Real-time detection of L-ascorbic acid and hydrogen peroxide in crude food samples employing a reversed sequential differential measuring technique of the SIRE-technology based biosensor[J]. Biosensors & Bioelectronics, 2001, 16: 363-369.
[2] A. Gregorio Alapont, L. Lahuerta Zamora, J. Martínez Calatayud. Indirect determination of paracetamol in pharmaceutical formulations by inhibition of the system luminol–H2O2 –Fe(CN)63-chemiluminescence[J]. Journal of Pharmaceutical and Biomedical Analysis, 1999, 21: 311-317.
[3] Hongfei Yue, Xin Bu, Ming-Hsing Huang et al. Quantitative determination of trace levels of hydrogen peroxide in crospovidone and a pharmaceutical product using high performance liquid chromatography with coulometric detection[J]. International Journal of Pharmaceutics, 2009, 375: 33-40.
[4] Rosanna Toniolo, Paola Geatti, Gino Bontempelli et al. Amperometric monitoring of hydrogen peroxide in workplace atmospheres by electrodes supported on ion-exchange membranes[J]. Journal of Electroanalytical Chemistry, 2001, 514: 123-128.
[5] Fengxian Gao, Ruo Yuan, Yaqin Chai, Shihong Chen et al. Amperometric hydrogen peroxide biosensor based on the immobilization of HRP on nano-Au/Thi/poly ( p-aminobenzene sulfonic acid)-modified glassy carbon electrode[J]. Journal of Biochemical and Biophysical Methods, 2007, 70: 407-413.
[6] Xiaoyan Yang, Yingshu Guo, Zhenhua Mei. Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads[J]. Analytical Biochemistry, 2009, 393: 56-61.
[7] Megan Tarvina, Bruce McCordb, Kelly Mountc et al. Optimization of two methods for the analysis of hydrogen peroxide: High performance liquid chromatography with ?uorescence detection and high performance liquid chromatography with electrochemical detection in direct current mode[J]. Journal of Chromatography A, 2010, 1217: 7564-7572.
[8] YUAN Jinchun, SHILLER A M. Determination of subnanomolar levels of hydrogen peroxide in seawater by reagent-injection chemiluminescence detection[J]. Anal Chem, 1999, 71(10): 1975-1980.
[9] 陈亚红，谢东坡，田丰收.不同供氢底物用于测定H2O2的酶催化动力学研究[J].分析测试学报，2007，26(6)：833-836.
[10] M.R. Guascitoa, E. Filippob, C. Malitesta et al. A new amperometric nanostructured sensor for the analytical determination of hydrogen peroxide[J]. Biosensors and Bioelectronics, 2008, 24: 1057-1063.
[11] 高风仙, 袁若, 柴雅琴等. 基于半胱氨酸/PDDA/纳米金共修饰的过氧化氢生物传感器研究[J]. 化学传感器, 2006, 26(3): 24-27.
[12] Cun-Xi Lei, Shun-Qin Huc, Na Gao et al. An amperometric hydrogen peroxide biosensor based on immobilizing horseradish peroxidase to a nano-Au monolayer supported by sol–gel derived carbon ceramic electrode. Bioelectrochemistry, 2004, 65: 33-39.
[13] Zhi-Min Liu, Yu Yang, Hua Wang. A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modi?ed gold electrode[J]. Sensors and Actuators B, 2005, 106: 394-400.
[14] Ai Wu Shi, Feng Li Qu, Ming Hui Yang et al. Amperometric H2O2 biosensor based on poly-thionine nanowire/HRP/nano-Au-modi?ed glassy carbon electrode[J]. Sensors and Actuators B, 2008, 129: 779-783.
[15] Soundappan Thiagarajan, Buo-Wei Su, Shen-Ming Chen. Nano TiO2–Au–KI ?lm sensor for the electrocatalytic oxidation of hydrogen peroxide[J]. Sensors and Actuators B: Chemical, 2009, 136: 464-471.
[16] CHEN Xianlan, PAN Hiabo, LIU Hongfang, et al. Nonenzymatic glucose sensor based on ?ower-shaped Au@Pd core-shell nanoparticles-ionic liquids composite ?lm modi?ed glassy carbon electrodes[J]. Electrochimica Acta, 2010, 56(2): 636-643.
[17] LIU Tongshen, KANG Tianfang, LU Liping, et al. Au–Fe(III) nanoparticle modi?ed glassy carbon electrode for electrochemical nitrite sensor[J]. J Electroanal Chem, 2009, 632(1-2): 197-200.
[18] Shaojun Yao, Junhui Xu, Ying Wang et al. A highly sensitive hydrogen peroxide amperometric sensor based on MnO2 nanoparticles and dihexadecyl hydrogen phosphate composite ?lm[J]. Analytica Chimica Acta, 2006, 557: 78-84.
[19] Abdollah Noorbakhsh, Abdollah Salimi. Amperometric detection of hydrogen peroxide at nano-nickel oxide/thionine and celestine blue nanocomposite-modi?ed glassy carbon electrodes[J]. Electrochimica Acta, 2009, 54: 6312-6321.
[20] 邓子峰, 张洪伟, 焦玉珠. 基于细胞色素 C /氧化锌的过氧化氢生物传感器[J]. 分析化学研究简报, 2009, 37(4): 613-616.
[21] 阳明辉, 李春香, 杨云慧等. 基于静电吸附多层膜固定酶的过氧化氢生物传感器的研究[J]. 化学学报, 2004, 62(5): 502-507.
[22] 李文娟, 袁若, 柴雅琴等. 基于层层静电吸附天青Ⅰ和纳米金固定过氧化物酶生物传感器的研究[J]. 分析试验室, 2007, 26(10): 9-13.
[23] 郭鶴桐，姚素薇，基础电化学及其测量[B]. 化学工业出版社, 2009.