Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201308031

Abstract

Abstract: In this work, tea saponin was purified by reversed-phase ODS-A column chromatography from degreased coldpressed tea seed meal for use as a reference standard in the quantitative determination of tea saponin by HPLC and identified by UV absorption spectrometry, FTIR and mass spectrometry. HPLC separation was achieved with reversed-phase ODS column and acetonitrile-water as eluent (an initial acetonitrile concentration of 20% held for 5 min, then increased to 100% in 5 min, and finally decreased back to the initial value) at a flow rate of 1.0 mL/min in gradient mode. UV detection was carried out at 215 nm. Under these conditions, symmetric peaks, desired retention time and good separation between tea saponin and impurity were observed.

Key words: high performance liquid chromatography (HPLC), cold-pressed tea seed meal, tea saponin, detection

CLC Number:

TS229

ZHANG Hai-long，ZHANG Wei-nong，HU Zhi-xiong，QI Yu-tang，GONG Ren. Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2013, 34(8): 153-156.

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