| 参考文献: [1] GRIFFIN P M, TAUXE R V. The epidemlology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome[J]. Epidemiologic Reviews, 1991, 13: 60-98.[2] ONG J, ZHE L, ROBINS-BPOWNE R, et al. Prevalence of verocytotoxigenic Escherichia coil serotype O157: H7 in children with diarrhoea attending a Sydney hospital[J]. Journal of Paediatrucs and Child Health, 1993, 29(3): 185-187.[3] SCOTTER S, ALDRIDGE M, CAPPS K. Validation of a method for the detection of E. coli O157:H7 in foods[J]. Food Control, 2000, 11(2):85-95.[4] 中华人民共和国卫生部. GB/T 4789.36-2008 食品卫生微生物检验大肠埃希氏菌O157：H7/NM检验[S]. 北京:中国标准出版社，2008.[5] 李文涛, 王俊东, 杨利峰, 等. 实时荧光定量 PCR技术及其应用[J]. 生物技术通讯, 2006, 17(1):112-114.[6] VERDERIO P, PIZZAMIGLIO S, GALLO F, et al. FCI: an R-based algorithm for evaluating uncertainty of absolute real-time quantification[J]. BMC Bioinformatics, 2008, 9:13.[7] THOMAS D S, KENNETH J L. Analyzing real-time PCR data by the comparative CT method[J]. Nature Protocols,2008, 3(6):1101-1108.[8] LEE J L, LEVIN R E. Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time PCR without enrichment[J]. Food Microbiology, 2011, 28(3):562-567.[9] CAREY C M, KOSTRZYNSKA M, THOMPSON S. Escherichia coli O157:H7 stress and virulence gene expression on romaine lettuce using comparative real-time PCR[J]. Journal of Microbiological Methods, 2009, 77(2): 235-242.[10] YOSHITOMI K J , JINNEMANK C, WEAGANT S D. Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR[J]. Molecular and Cellular Probes, 2006, 20(1): 31-41.[11] CHASSAGNE L, PRADEL N, ROBIN F, et al. Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR green in a real-time polymerase chain reaction[J]. Diagnostic Microbiology and Infectious Disease, 2009, 64(1):98-101.[12] 胡 慧，陈雅君，段志刚，等.大肠杆菌O157:H7特异基因的实时荧光定量PCR检测[J]. 食品科学, 2011, 32(12):278-282.[13] WANG YUEXIA, SUO Biao. A new 7-plex PCR assay for simultaneous detection of shiga toxin-producing Escherichia coli O157 and Salmonella Enteritidis in meat products [J]. Journal Für Verbraucherschutz Und Lebensmittelsicherheit, 2011,6(4):441-447.[14] WEAGANT S D, JINEMAN K C, YOSHITOMI K J, et al. Optimization and evaluation of a modi?ed enrichment procedure combined with immunomagnetic separation for detection of E. coli O157:H7 from arti?cially contaminated alfalfa sprouts[J]. International Journal of Food Microbiology, 2011, 149(3):209-217.[15] FU Z, ROGELJ S, KIEFT T L. Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR[J]. International Journal of Food Microbiology, 2005, 99(1):47-57.[16] NGUYEN L T, GILLESPIE B E, NAM H M, et al. Detection of Escherichia coil O157:H7 and Listeria monocytogenes in beef products by real-time polymerase chain reaction[J]. Foodborne Pathogens and Disease, 2004, 1(4):231-240.[17] KAWASAKI S, FRATAMICO P M, HORIKOSHI N, et al. Multiplex real-time polymerase chain reaction assay for simultaneous detection and quanti?cation of Salmonella species, Listeria monocytogenes, and Escherichia coli O157:H7 in ground pork samples[J]. Foodborne Pathpgns and Disease, 2010, 7(5): 549-554.[18] SHARMA VK. Real-time reverse transcription-multiplex PCR for simultaneous and speci?c detection of rfbE and eae genes of Escherichia coli O157:H7[J]. Molecular and Cellular Probes, 2006, 20(5):298-306.[19] SUO B, HE Y, TU SI, et al. A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp, Escherichia coli O157, and Listeria monocytogenes in meat products[J]. Foodborne Pathogens and Disease, 2010, 7(6): 619-6-28.[20] JINNEMAN K C, YOSHITOMI K J, WEAGANT S D. Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype[J]. Applied and Environmental Microbiology, 2003, 69(10): 6327-6333.[21] SHARMA V K, DEAN-NYSTROM E A. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins[J]. Veterinary Microbiology, 2003, 93(3): 247-260.[22] SANDHYA S, CHEN W. MULVHANDANI A. Molecular beacons: A real-time polymerase chain reaction assay for detecting Escherichia coli from fresh produce and water[J]. Analytica Chimica Acta, 2008, 614(2):208-212.[23] SINGH J, BATISH VK, GROVER S. A molecular beacon-based duplex real-time polymerase chain reaction assay for simultaneous detection of Escherichia coli O157:H7 and Listeria monocytogenes in milk and milk product[J]. Foodborne Pathogens and Disease, 2009, 6(10): 1195-1201.[24] FORTIN N Y, MULCHANDANI A, CHEN W. Use of real-time polymerase chain reaction and nolecular beacons for the detection of Escherichia coli O157:H7[J]. Analytical Biochemistry, 2001, 289(2): 281-288.[25] KONTANIS E J, REED F A, et al. Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors[J]. Journal of Forensic Sciences, 2006, 51(4):795-804.[26] YANG Xianqin, BADONI M, GILL C O. Use of propidium monoazide and quantitative PCR for differentiation of viable Escherichia coli from E. coli killed by mild or pasteurizing heat treatments[J]. Food Microbiology, 2011,28(8):1478-1482.[27] CONTRERAS P J, URRUTIA H, SOSSA K, et al. Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment[J]. Journal of Microbiological Methods,2011,87(1):89-95.[28] L?VAL T, HOVDA M B, BJ?KBLOM B. Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua[J]. Journal of Microbiological Methods, 2011,85(2):164-169.[29] SLIMANI S, ROBYNS A, JARRAUD S, et al. Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR[J]. Journal of Microbiological Methods, 2012,88(2):319-321.[30] FEDIO W M, JINNEMAN K C, YOSHITOMI K J, et al. Detection of E. coli O157:H7 in raw ground beef by PathatrixTM immunomagnetic-separation, real-time PCR and cultural methods[J]. International Journal of Food Microbilogy, 2011,148(2):87-92. |
