Abstract:

We developed a loop-mediated isothermal amplification (LAMP) method for the detection of Bt gene in cooked
rice and rice wine. Bt gene (GeneBank accession number Y09787) was amplified by a set of four special primers that
were designed according to six regions of the target gene. We optimized the reaction conditions Mg2+ concentration, Bst
DNA polymerase concentration, temperature and time. We also explored the specificity and sensitivity of the reaction. The
results showed that the LAMP reaction system allowed easy and effective detection of Bt gene with a high specificity and
sensitivity. The limit of detection was 0.005%. The LAMP detection method is specific, sensitive and reliable without need
for special instruments.

Key words: Bt gene, loop-mediated isothermal amplification (LAMP), detection, rice products