Abstract:

The community compositions of bacteria and archaea in mature pit mud of Lu-flavor liquor were detected
through establishing 16S rRNA gene clone library of bacteria and archaea, sequencing and analyzing phylogenetic tree. Data
analysis showed that the biodiversity of bacteria in pit mud was quite rich, reaching 34 OTUs, which were mainly distributed
in 14 families and 2 unidentified families of Clostridia and Bacteroidia. And also, most of them were restricted anaerobes.
Among them, Clostridia strains were absolutely dominant in the bacterial community of pit mud, and its ratio of clone
number was 68.2%. In addition, there was appropriate distribution for Bacilii (21.6%), Bacteroidetes (8.0%), Tenericutes
(1.1%) and Synergistetes (1.1%). Archaeal community composition was quite simple, and only 4 OTUs were detected
and mainly distributed in Methanoculleus, Methanosarcina, Methanobacterium and Methanomassiliicoccus with clone
distribution ratios of 58.8%, 29.4%, 5.9% and 5.9%, respectively.

Key words: Lu-flavor liquor, pit mud, culture-independent approach, bacteria, archaea, 16S rRNA gene, clone library