FOOD SCIENCE

• Analysis & Detection • Previous Articles     Next Articles

Detection of Cat-Derived Components in Foods by Real-Time Polymerase Chain Reaction

GAO Xiao-li1, YANG Xin-ting2, WANG Dan2, ZHAO Lin-na2, CHEN Ji-han1, HOU Cai-yun1,*   

  1. 1. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality,
    College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: HOU Cai-yun

Abstract:

In this study, a TaqMan-based, sensitive and specific real-time polymerase chain reaction (PCR) assay was
developed for the detection of cat-derived components in foods. Primers and TaqMan probe were designed based on the
conserved sequence of the feline mitochondrial ND1, and the performance of the method was invalidated. Results indicated
that no cross-reaction was observed between the feline and non-target species, and this method revealed a high sensitivity and
could detect 1 pg of cat template DNA. In conclusion, the TaqMan probe assay used in this study can be a rapid and sensitive
method for the routine identification of meat species in foods.

Key words: real-time PCR, mitochondrial ND1, cat-origin component

CLC Number: