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Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788

LIU Xin1, ZHANG Guocong2, ZHOU Xinghu3, ZHU Changqing1, HUANG Ming1,*   

  1. 1. Nanjing Innovation Center of Meat Products Processing, College of Food Science and Technology,Nanjing Agricultural University, Nanjing 210095, China; 2. Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041, China; 3. Nanjing Jiabang Food Co. Ltd., Nanjing 211225, China
  • Online:2015-02-25 Published:2015-02-16
  • Contact: HUANG Ming

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Abstract:

For implementation of labeling regulations, an event-specific real-time PCR method for the detection of
genetically modified soybean MON89788 was established in this study. Primers and TaqMan probe were designed based on
the event-specific sequence of MON89788 soybean. The specificity, sensitivity and repeatability of the developed method
were examined, respectively. The results showed that the method could amplify a 127 bp product specifically; the limit of
detection was 0.1% in 50 ng of soybean genomic DNA, approximately corresponding to 40 soybean haploid genome copies;
and this method had good repeatability and could be successfully applied to the detection of real samples. These results
indicated that the strain-specific real-time PCR method was suitable for the identification of genetically modified soybean
MON89788 and its products.

Key words: genetically modified soybean, MON89788 event, real-time PCR, event-specific detection, transgenic labeling

CLC Number: