FOOD SCIENCE

Previous Articles     Next Articles

Event-specific Detection of Genetically Modified Rice Using Enzyme-Linked Probe Hybridization Chip

ZHANG Ming-zhe1, CHEN Wu-jian1, ZHANG Xiao-feng2, CHEN Xiao-mei2, CHEN Xi1, WU Rong3   

  1. 1. Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China;
    2. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;
    3. Hangzhou Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310012, China
  • Online:2014-04-25 Published:2014-05-13

RichHTML

0

PDF (PC)

171

Abstract:

The enzyme-linked probe hybridization chip is a method based on ligase-hybridizing probe chip technology with
the principle of using thio-primers for protection, and using Lambda DNA exonuclease to cut multiple PCR amplicons into
single products. 5’-End probe chain is fixed in the amino-modified chip, and hybridized with the single-chain product by
adding the fluorescent-modified probe for the enzyme-linked reactions with the anterior probe in order to achieve highly
specific, parallel and high-throughput detection. In this study, the enzyme-linked probe hybridization chip could effectively
detect genetically modified rice with high specificity and the sensitivity limit was 0.1%. This chip can be used by the entryexit
inspection and quarantine authorities and be applied for food safety testing.

Key words: enzyme-linked probe hybridization chip, genetically modified rice, event-specific detection

CLC Number: