食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 176-180.doi: 10.7506/spkx1002-6300-200918037

• 工艺技术 • 上一篇    下一篇

微波法与超声破碎法提取蛹虫草菌丝体中虫草素的比较研究

黄子琪1,贲松彬1,桑育黎2,代 颖2,李慧娇2,陈长兰2 ,*   

  1. 1.辽宁大学生命科学院 2.辽宁大学药学院
  • 收稿日期:2009-06-22 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 陈长兰 E-mail:chenchanglanbio@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(30671176);辽宁省教育厅重点实验室项目(2009S046);沈阳市人才开发专项资金项目(2008040403035)

Extraction of Cordycepin from Fermented Mycelia of Cordyceps militaris: A Comparitive Study of Ultrasonic-assisted and Microwave-assisted Procedures

HUANG Zi-qi1,BEN Song-bin1,SANG Yu-li2,DAI Ying2,LI Hui-jiao2,CHEN Chang-lan2,*   

  1. 1. School of Life Science, Liaoning University, Shenyang 110036, China;
    2. School of Pharceutical Science, Liaoning University, Shenyang 110036, China
  • Received:2009-06-22 Online:2009-09-15 Published:2010-12-29
  • Contact: CHEN Chang-lan E-mail:chenchanglanbio@yahoo.com.cn

摘要:

分别就微波法和超声破碎法用于蛹虫草菌丝体中虫草素提取时应采取的工艺条件进行研究。采用单因素分析和正交设计试验分别研究提取溶剂、提取液pH 值、微波和超声波的功率、提取时间对虫草素提取得率的影响。正交试验结果表明,微波提取法要好于超声波提取法。当以提取溶剂为水- 乙醇(1:3,V/V)、pH7.0、微波功率500W,并提取3min 的条件进行微波提取时,虫草素的提取得率最高。

关键词: 虫草素, 提取, 微波, 超声波, 正交设计

Abstract:

Ultrasonic and microwave techniques were separately used to assist the extraction of cordycepin from fermented mycelia of Cordyceps militaris with ethanol. On the basis of single factor experiments, ultrasonic-assisted and microwave-assisted extraction procedures were optimized for maximizing yield of cordycepin using L9(34) orthogonal array design. The results showed that the assisting effect of microwave was better than that of ultrasonic, and a maximum yield of cordycepin was obtained under the following extraction conditions: microwave power, 500 W; extraction solvent, a mixture of water and absolute ethanol (1:3, V/V) at pH 7.0; and extraction time 3 min.

Key words: cordycepin, extraction, microwave, ultrasonic, orthogonal design

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