食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 220-222.doi: 10.7506/spkx1002-6300-200920045

• 工艺技术 • 上一篇    下一篇

酶法制备芝麻粕多肽的工艺研究

王连翠   

  1. 临沂师范学院生命科学学院
  • 收稿日期:2009-07-26 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 王连翠 E-mail:weid1128@163.com
  • 基金资助:

    国家星火计划项目(2008GI690001)

Preparation of Polypeptides from Sesame Meal by Enzymatic Hydrolysis

WANG Lian-cui   

  1. (College of Life Science, Linyi Normal University, Linyi 276005, China)
  • Received:2009-07-26 Online:2009-10-15 Published:2010-12-29
  • Contact: WANG Lian-cui E-mail:weid1128@163.com

摘要:

通过单因素试验和正交试验分析,确定碱性蛋白酶Alcalase水解芝麻粕蛋白的最佳水解工艺条件为底物浓度70g/L、温度65℃、[E]/[S]为6%、pH8.0、水解时间3h。在此水解条件下,水解液水解度达到了31.3%。

关键词: 芝麻粕蛋白, 水解度, 多肽, 酶解

Abstract:

The optimal technological parameters of alcalase-catalyzed hydrolysis of proteins in sesame meal, including temperature, pH, and reaction time were investigated by single factor method and orthogonal array design. Results showed that a maximum hydrolysis degree of soybean proteins of 31.3% was obtained through the optimal hydrolysis procedure conducted for 3 h with a substrate concentration of 60 g/L and an [E]/[S] ratio of 6% at 65 ℃ and pH 8.0.

Key words: sesame meal, hydrolysis degree, polypeptide, enzymatic hydrolysis

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