亚临界水萃取大孔树脂分离纯化制备紫草素

doi:10.7506/spkx1002-6300-201008001

食品科学 ›› 2010, Vol. 31 ›› Issue (8): 1-5.doi: 10.7506/spkx1002-6300-201008001

• 工艺技术 • 下一篇

亚临界水萃取大孔树脂分离纯化制备紫草素

黄萍萍¹，杨日福²，黄星歆¹，范晓丹¹，丘泰球^{1 ,*}

1.华南理工大学轻工与食品学院 2.华南理工大学物理科学与技术学院

收稿日期:2009-06-12 出版日期:2010-04-15 发布日期:2010-12-29
通讯作者: 丘泰球 E-mail:tqqiu@scut.edu.cn
基金资助:
国家自然科学基金项目(20776047)；“十一五”国家科技支撑计划项目(2006BAD27B03)

Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma

HUANG Ping-ping1，YANG Ri-fu2，HUANG Xing-xin1，FAN Xiao-dan1，QIU Tai-qiu1,*

1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China；
2. School of Physics, South China University of Technology, Guangzhou 510640, China

Received:2009-06-12 Online:2010-04-15 Published:2010-12-29
Contact: QIU Tai-qiu1 E-mail:tqqiu@scut.edu.cn

摘要/Abstract

摘要：

以新疆紫草为研究对象，考察亚临界水的动态循环和静态平衡两种萃取方式对紫草素提取率的影响。选取不同极性的大孔树脂和有机溶剂对提取液中的紫草素进行吸附、解析实验；使用高效液相- 蒸发光散射(HPLCELSD)技术分析样品的纯度。结果显示：10MPa、120℃、1.39cm3/s 的流量，动态循环萃取8min，紫草素提取率最高是0.216%；静态平衡萃取16min，紫草素提取率最高是0.198%。DA-201 大孔树脂对紫草素的吸附效果最佳，恒温摇床振荡吸附24h，吸附率是89.7%；丙酮的解析效果最好，能够将93.2% 的紫草素解析出来，且速率较快。经大孔树脂吸附分离后，紫草素的纯度由27.31% 提高至85.07%。实验表明，亚临界水萃取、大孔树脂吸附分离能够有效制备纯度较高的紫草素。

关键词: 亚临界水, 动态循环, 静态平衡, 紫草素, 大孔树脂

Abstract:

To prepare high-purity shikonin from Arnebia euchroma grown in Xinjiang autonomous region, subcritical water extraction and macroporous resin adsorption for purification were conducted. Effects of two extraction modes, namely dynamic circulation and static equilibrium, on shikonin yield were examined. Shikonin adsorption/desorption experiments were carried out using different polar macroporous resins and organic solvents. HPLC-ELSD was used to analyze the purity of the product obtained. Results showed that a maximum shikonin yield of 0.216% was achieved through 8 min extraction in the mode of dynamic circulation under the following conditions: extraction pressure 10 MPa, extraction temperature 120 ℃ and flow rate 1.39 cm3/s; in contrast, the 16 min static equilibrium extraction gave a maximum shikonin yield of 0.198%. Among 5 macroporous resins tested, DA-201 resin exhibited the best adsorption performance and an adsorption rate of 89.7% was obtained after 24 h constant temperature shaking adsorption. Among 4 organic solvents tested, acetone was the best one for shikonin desorption and desorbed 93.2% of shikonin at a fast velocity. The purity of shikonin was increased from 27.31% to 85.07% after optimized purification using DA-201 resin and acetone. The above results demonstrate that subcritical water extraction followed by macroporous resin adsorption provides an effective approach to preparing high-purity shikonin from Arnebia euchroma.

Key words: sub-critical water, dynamic circulation, static equilibrium, shikonin, macroporous resin

中图分类号:

TQ645.96

黄萍萍1，杨日福2，黄星歆1，范晓丹1，丘泰球1,*. 亚临界水萃取大孔树脂分离纯化制备紫草素[J]. 食品科学, 2010, 31(8): 1-5.

HUANG Ping-ping1，YANG Ri-fu2，HUANG Xing-xin1，FAN Xiao-dan1，QIU Tai-qiu1,*. Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma[J]. FOOD SCIENCE, 2010, 31(8): 1-5.

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亚临界水萃取大孔树脂分离纯化制备紫草素

Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma

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