食品科学 ›› 2009, Vol. 30 ›› Issue (6): 180-183.doi: 10.7506/spkx1002-6630-200906040

• 分析检测 • 上一篇    下一篇

畜禽肌肉中肌苷酸测定的高效毛细管电泳法研究

马 旺1,蔡海莹1,*,檀华蓉2,王艳红2   

  1. 1.安徽农业大学动物科技学院 2.安徽农业大学生物技术中心
  • 收稿日期:2008-04-28 修回日期:2008-06-30 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 蔡海莹 E-mail:haiying20@anau.edu.cn
  • 基金资助:

    安徽省教育厅自然科学基金项目(2006KJ216B)

Determination of Inosinic Acid in Animal Muscles by High Performance Capillary Electrophoresis

MA Wang1,CAI Hai-ying1,*,TAN Hua-rong2,WANG Yan-hong2   

  1. (1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. Biotechnology Center, Anhui Agricultural University, Heifei 230036, China)
  • Received:2008-04-28 Revised:2008-06-30 Online:2009-03-15 Published:2010-12-29
  • Contact: CAI Hai-ying1,*, E-mail:haiying20@anau.edu.cn

摘要:

本实验对毛细管电泳(CE)测定畜禽肌肉中肌苷酸含量方法的建立进行了研究。采用P/ACETM MDQ 高效毛细管电泳仪,电泳缓冲液为20mmol/L 磷酸氢二钾- 四硼酸钠,pH6.5,电泳温度为25℃,电压为22kV,紫外检测波长为248nm。结果表明,肌肉中肌苷酸含量测定结果相对标准偏差为1.72%,后加标准品和前加标准品回收率分别为80.91%~84.14% 和79.03%~82.41%。该方法方便、快捷,对畜禽肌肉风味物质肌苷酸含量测定准确度良好,是值得推广的一种新方法。

关键词: 毛细管电泳, 肌苷酸, 肌肉

Abstract:

The aim of this study was to set up a capillary electrophoresis method for determining inosinic acid in animal muscles. P/ACETM MDQ high performance capillary electriphoresis (HPCE) instrument was adopted. The buffer, pH value, temperature, oltage and UV detection wavelength of HPCE were 20 mmol/L K2HPO4-NaB4O7·10H2O, 6.5, 25 ℃, 22 kV and 248 nm, espectively. The results showed that the relative standard deviation of inosinic acid in animal muscle is 1.72% and its recoveries f retro-plus and pro-plus standard preparations are 80.91% to 84.14% and 79.03% to 82.41%. This new method is convenient, apid and accurate for assaying inosinic acid in muscle flavor substance and deserved to be spread.

Key words: capillary electrophoresis, nosinic acid, muscle

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