关键词: 猪血浆蛋白, 水解, 抗氧化性

Abstract:

Porcine plasma protein (4%, w/w) was hydrolyzed by alcalase for 0.5 h to 6 h, and the degree of hydrolysis (DH) was determined by pH-Stat method. Meanwhile, reducing power, metal-chelating (Cu2+ and Fe2+) activity and DPPH radicals scavenging activity of hydrolysates were also determined. The results showed that the products of 5h-hydrolysis have good reducing power, metal ion chelating activity and DPPH radical scavenging activity. Compared among different concentrations of hydrolysates, the antioxidant activity significantly increases (p＜0.05) with the increasing of concentration. Although intact porcine plasma protein also displays the antioxidation, it is far less potent than hydrolyzed porcine plasma protein (p＜0.05). In conclusion, enzyme-hydrolyzed porcine plasma protein can act as a metal ion chelator, a hydrogen donor, as well as a radical stabilizer to inhibit lipid oxidation because of its antioxidant activity.

Key words: porcine plasma protein, hydrolysis, antioxidant activity