食品科学 ›› 2009, Vol. 30 ›› Issue (7): 299-303.doi: 10.7506/spkx1002-6630-200907069

• 专题论述 • 上一篇    下一篇

16S rRNA序列分析及其在乳酸菌分类鉴定中的应用

赵玉娟,牛春华,张 雪,李 达,张 健,杨贞耐*   

  1. 吉林省农业科学院农产品加工中心
  • 收稿日期:2008-05-29 修回日期:2008-06-12 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 杨贞耐 E-mail:zyang@cjaas.com
  • 基金资助:

    科技部国际合作项目(2006DFA32620);吉林省科技厅重点项目(20060219)

Sequence Analysis of 16S rRNA and Its Application in Classification and Identification of Lactic Acid Bacteria

ZHAO Yu-juan,NIU Chun-hua,ZHANG Xue,LI Da,ZHANG Jian,YANG Zhen-nai   

  1. Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
  • Received:2008-05-29 Revised:2008-06-12 Online:2009-04-01 Published:2010-12-29
  • Contact: YANG Zhen-nai E-mail:zyang@cjaas.com

摘要:

综述16S rRNA的特点及序列分析的基本方法,并详细介绍了几种在乳酸菌分类和鉴定中较常用的16S rRNA相关的分子生物学方法,如16S rRNA直接测序、寡核苷酸探针、限制性片段长度多态性分析、温度梯度凝胶电泳等,以及这些方法的研究进展。

关键词: 乳酸菌, 16S rRNA, 分类, 鉴定

Abstract:

The characteristics of 16S rRNA and the fundamental methodology of its sequence analysis were reviewed in this paper. Several molecular biological methods related to 16S rRNA sequence analysis were detailed to classify and identify lactic acid bacteria such as direct sequencing, oligonucleotide probes, the polymorphism of restriction fragment length, temperature gradient gel electrophoresis. The recent progresses in the application of these methods were also introduced in detail.

Key words: lactic acid bacteria, 16S rRNA, classification, identification

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