UPLC -MS/MS法测定苹果中苯并咪唑类农药残留

doi:10.7506/spkx1002-6630-200912046

食品科学 ›› 2009, Vol. 30 ›› Issue (12): 209-212.doi: 10.7506/spkx1002-6630-200912046

UPLC -MS/MS法测定苹果中苯并咪唑类农药残留

赵领军¹，岳晖²，郭栋梁²，崔锦³，赵善仓²

1.济南市农业信息中心 2.农业部食品质量监督检验测试中心(济南) 3.莱芜市质量检验所

收稿日期:2008-09-05 修回日期:2008-12-12 出版日期:2009-06-15 发布日期:2010-12-29
通讯作者: 赵领军 E-mail:lingly_zhao@yahoo.com

Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Benzimidazoles Residues in Apples

ZHAO Ling-jun1，YUE Hui2，GUO Dong-liang2，CUI Jin3，ZHAO Shan-cang2

(1. Jinan Agriculture Information Center, Jinan 250002, China；2. Inspection Center of Food Quality (Jinan), Ministry of
Agricalture, Jinan 250100, China；3. Laiwu Product Quality Supervision and Inspection Institute, Laiwu 271100, China)

Received:2008-09-05 Revised:2008-12-12 Online:2009-06-15 Published:2010-12-29
Contact: ZHAO Ling-jun1， E-mail:lingly_zhao@yahoo.com

摘要/Abstract

摘要：

建立超高效液相色谱串联质谱法(UPLC-MS/MS)测定苹果中苯并咪唑类残留的快速检测方法。样品经乙腈提取后盐析，过0.2μm 滤膜，电喷雾电离，正离子模式，多反应检测(MRM)。多菌灵、噻菌灵、甲基硫菌灵的检出限分别为0.17、0.16、0.07μg/kg，添加回收率的范围89.2%～104.6%，相对标准偏差为1.7%～3.6%。本方法快速、易操作、灵敏度高，完全满足农药残留检测的需要。

关键词: UPLC-MS/MS, 苹果, 苯并咪唑, 残留

Abstract:

Objective: To establish a method for determination of benzimidazoles residues in apples by UPLC-MS/MS. Methods: Sample was extracted with acetonitrile. After impurity removal by NaCl salting out, the sample was filtered through a membrane (0.2 μm), and injected into UPLC column. Identification was achieved by electrospray ionization in positive mode (ESI+) using multiple reaction monitoring (MRM). Results: The detection limits for carbendazim, thiabendazol, thiophanatemethyle were 0.17, 0.16, 0.07 μg/kg, respectively; the average recoveries for the three benzimidazoles in blank sample spiked at three levels (1.0, 5.0 and 10.0 μg/kg) were in the range of 89.2%－104.6%, and the RSDs were 1.7%－3.6% (n = 6). Conclusion: The method can completely meet the requirement of analysis for benzimidazoles residues in apples.

Key words: UPLC-MS/MS, apple, benzimidazole, residue

中图分类号:

TS207.53

赵领军1，岳晖2，郭栋梁2，崔锦3，赵善仓2. UPLC -MS/MS法测定苹果中苯并咪唑类农药残留[J]. 食品科学, 2009, 30(12): 209-212.

ZHAO Ling-jun1，YUE Hui2，GUO Dong-liang2，CUI Jin3，ZHAO Shan-cang2. Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Benzimidazoles Residues in Apples[J]. FOOD SCIENCE, 2009, 30(12): 209-212.

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UPLC -MS/MS法测定苹果中苯并咪唑类农药残留

Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Benzimidazoles Residues in Apples

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