食品科学 ›› 2009, Vol. 30 ›› Issue (14 ): 140-142.doi: 10.7506/spkx1002-6630-200914024

• 工艺技术 • 上一篇    下一篇

菱角壳总黄酮超声辅助提取工艺研究

李维莉,马银海,张亚平,杨秋萍,黄椿淋   

  1. 昆明学院化学科学与技术系
  • 收稿日期:2009-04-14 出版日期:2009-07-15 发布日期:2010-12-29
  • 通讯作者: 李维莉 E-mail:lierkm@163.com

Ultrasound-assisted Extraction of Total Flavonoids from Water Chestnut Hull

LI Wei-li,MA Yin-hai,ZHANG Ya-ping,YANG Qiu-ping,HUANG Chun-lin   

  1. (Department of Chemical Science and Technology, Kunming University, Kunming 650031, China
  • Received:2009-04-14 Online:2009-07-15 Published:2010-12-29
  • Contact: LI Wei-li, E-mail:lierkm@163.com

摘要:

目的:确定菱角壳中总黄酮的优化超声回流提取工艺条件。方法:采用正交试验法考察溶剂用量、溶剂浓度、提取次数、提取时间对总黄酮提取率的影响,用标准曲线法测定总黄酮含量。结果:在超声提取所考察的因素中,采用50% 的乙醇、取液料比10:1(V/m),超声提取2 次、每次提取0.5h,在此条件下黄酮提取可达0 . 81%。结论:该方法具有操作简单、提取时间短、提取率高等优点。

关键词: 菱角壳, 总黄酮, 超声辅助提取

Abstract:

Ultrasonic technique was employed to extract total flavonoids from water chestnut hull with ethanol. Effects of four crucial factors including ethanol concentration, liquid/material ratio (V/m), extraction duration and repeated extraction times on extraction yield of total flavonoids were investigated by one-factor-at-a-time method. Subsequently, all these factors were optimized using orthogonal array design. The optimal parameters for extracting total flavonoids were as follows: ethanol concentration 50% and liquid/material ratio 10:1 for an extraction duration of 0.5 h repeated twice, and the yield of total flavonoids was up to 0.81% under these optimized conditions. The ultrasonic-assisted extraction for total flavonoids in water chestnut hull is a time- and cost-saving and highly efficient method.

Key words: water chestnut hull, total flavonoids, ultrasonic-assisted extraction

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