关键词: 环糊精葡萄糖基转移酶, 工艺条件, 优化

Abstract:

A high-yield Bacillus strain producing cyclodextrin glycosyltransferase (CGTase) has been screened and bred by our lab. In the present study, one-factor-at-a-time method was adopted to investigate the effects of medium components and fermentation conditions on CGTase production by the strain. The results showed that the optimal carbon source, nitrogen source and carbonate for the growth of the strain were 1% soluble starch, 0.1% yeast extract and 1% Na2CO3, respectively. And the optimal fermentation parameters were determined as follows: initial pH of fermentation medium 10, fermentation temperature 33 ℃, inoculation proportion 3%, rotation speed of shaker 180 r/min. A CGTase activity of 2842 U/ml was achieved under these conditions.

Key words: cyclodextrin glycosyltransferase, fermentation conditions, optimization