食品科学 ›› 2009, Vol. 30 ›› Issue (17): 30-33.doi: 10.7506/spkx1002-6630-200917004

• 基础研究 • 上一篇    下一篇

伴大豆球蛋白及其水解肽对沙门氏菌生长的影响

郭成东,张源淑,王国杰,邹思湘*   

  1. 南京农业大学 农业部动物生理生化重点开放实验室
  • 收稿日期:2008-08-25 修回日期:2009-02-13 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 邹思湘
  • 基金资助:

    教育部博士点基金资助项目(20060307007)

Effects of Conglycinin and Its Hydrolysate Peptide on Propagation of Salmonella

GUO Cheng-dong,ZHANG Yuan-shu,WANG Guo-jie,ZOU Si-xiang*   

  1. Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing
    Agricultural University, Nanjing 210095, China
  • Received:2008-08-25 Revised:2009-02-13 Online:2009-09-01 Published:2014-04-14
  • Contact: ZOU Si-xiang

摘要:

为了进一步研究伴大豆球蛋白的生物学功能,应用等电点沉淀和盐析的方法制备伴大豆球蛋白,通过凝胶柱层析法进一步分离纯化,得到单一组分,SDS-PAGE 电泳鉴定为纯化的伴大豆球蛋白。并对分离得到的伴大豆球蛋白及其胃蛋白酶水解肽对沙门氏菌生长的影响进行观察。结果表明:高浓度(6mg/ml)的伴大豆球蛋白及其水解多肽可以抑制沙门氏菌的生长繁殖,表现一定的抗沙门氏菌增殖的作用,同样浓度水解多肽的抑制作用强于伴大豆球蛋白本身;低浓度(1.5mg/ml及0.75mg/ml)的伴大豆球蛋白及其水解多肽则表现出轻微的促沙门氏菌增殖的作用。

关键词: 伴大豆球蛋白, 胃蛋白酶, 水解肽, 沙门氏菌

Abstract:

Conglycinin, one of the main storage proteins in soybean, was prepared by isoelectric precipitation and salting-out followed by gel column chromatographic purification. SDS-PAGE analysis clearly indicated that the purification product was electrophoretically pure. The effects of conglycinin and its hydrolysate peptide prepared with pepsin on propagation of Salmonella were studied. The propagation of Salmonella was inhibited by high concentration (6 mg/ml) of conglycinin and its hydrolysate peptide and the peptide exhibited stronger inhibition effect than conglycinin at the same concentration. However, both conglycinin and its hydrolysate peptide at low concentration (1.5 and 0.75 mg/ml) displayed slight promotion effects.

Key words: conglycinin, pepsin, hydrolysate peptide, Salmonella

中图分类号: