食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 397-400.doi: 10.7506/spkx1002-6630-200924090

• 分析检测 • 上一篇    下一篇

葡聚糖凝胶过滤色谱柱法测定两种氨基酸锌螯合物螯合率的差异性

胡晓波1,龚 毅1,郭智勇1,2,聂少平1,李 昌1,王远兴1,谢明勇1 ,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2.中国人民大学环境学院
  • 收稿日期:2009-09-10 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 谢明勇1 ,* E-mail:myxie@ncu.edu.cn
  • 基金资助:

    教育部“长江学者和创新团队发展计划”项目(IRT0540);2006 年度中国江西留学人员创业园科研项目

Chelation Rate of Zinc Amino Acid Chelate Determined by Sephadex Gel Filtration Chromatography

HU Xiao-bo1,GONG Yi1,GUO Zhi-yong1,2,NIE Shao-ping1,LI Chang1,WANG Yuan-xing1,XIE Ming-yong1,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. School of Environment and Natural Resources, Renmin University of China, Beijing 100872, China)
  • Received:2009-09-10 Online:2009-12-15 Published:2010-12-29
  • Contact: XIE Ming-yong1,* E-mail:myxie@ncu.edu.cn

摘要:

微量元素氨基酸螯合物的螯合率常用来反映微量元素氨基酸螯合物的品质,采用葡聚糖凝胶过滤色谱柱法测定蛋氨酸锌、苏氨酸锌两种氨基酸锌螯合物的螯合率,发现该法测定结果与其他测定方法分析结果一致,表明此法适用于测定蛋氨酸锌的螯合率;但测定苏氨酸锌螯合物的螯合率时,测定结果与其他测定方法分析结果不一致,结合葡聚糖凝胶过滤色谱柱法测定过程,表明此法不适用于测定苏氨酸锌的螯合率。

关键词: 氨基酸锌螯合物, 螯合率, 葡聚糖凝胶过滤色谱柱法, 蛋氨酸锌, 苏氨酸锌

Abstract:

Chelation rates between trace elements and amino acids are usually used to evaluate chelate quality. In this paper, chelation rates of zinc methioninate and zinc threoninate were determined by Sephadex gel filtration chromatography. Results indicated that Sephadex gel filtration chromatography could be used to determine chelation rate of zinc methioninate due to the consistent result with other determination methods. However, it was not feasible to determine the chelation rate of zinc threoninate.

Key words: zinc amino acid chelate, chelation rate, Sephadex gel filtration chromatography, zinc methioninate, zinc threoninate

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