食品科学 ›› 2010, Vol. 31 ›› Issue (10): 1-5.doi: 10.7506/spkx1002-6630-201010001

• 工艺技术 •    下一篇

小麦麸皮淀粉的分离制备与特征分析

赵瑞昌,田益华,姜 东* ,戴廷波,荆 奇,曹卫星   

  1. 南京农业大学 江苏省信息农业高技术研究重点实验室,农业部南方作物生理生态重点开放实验室
  • 收稿日期:2009-07-24 出版日期:2010-05-15 发布日期:2010-12-29
  • 通讯作者: 姜东 E-mail:jiangd@njau.edu.cn
  • 基金资助:

    国家自然科学基金项目(30671216;30700483;30971734);江苏省自然科学基金项目(BK2008329);
    教育部新世纪优秀人才资助计划项目(06-0493);国家现代农业产业技术体系建设专项(nycytx-03);
    江苏省粮丰工程项目(BE2009426)

Enzymatic Preparation and Physico-chemical Characterization of Wheat Bran Starch

ZHAO Rui-chang,TIAN Yi-hua,JIANG Dong*,DAI Ting-bo,JING Qi,CAO Wei-xing   

  1. Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Hi-Tech Key Laboratory of Information
    Agriculture of Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-07-24 Online:2010-05-15 Published:2010-12-29
  • Contact: JIANG Dong E-mail:jiangd@njau.edu.cn

摘要:

利用中性蛋白酶制备小麦麸皮淀粉条件的基础上,进一步分析所制备小麦麸皮淀粉的形态及理化特性。结果表明:中性蛋白酶法制备小麦麸皮淀粉的最佳条件为中性蛋白酶质量浓度3g/L、反应温度45℃、反应时间90min,在此条件下,100g 麸皮的淀粉产量为14.86g、蛋白含量为0.25%;麸皮淀粉中蛋白含量和破损淀粉含量均较低,小淀粉粒体积分数为58.4%;与面粉淀粉相比,麸皮淀粉中直链淀粉含量和糊化值较低,膨胀势较高,淀粉粒的相对结晶度较小。

关键词: 淀粉, 小麦, 麸皮, 中性蛋白酶

Abstract:

Optimal neutrase hydrolysis of wheat bran for starch preparation was investigated using orthogonal array design. Along with this, the prepared starch was characterized physicochemically. Results showed that the neutrase hydrolysis reaction for 90 min at 45 ℃ and an enzyme dosage of 3 g/L resulted in an optimal compromise between high starch yield and low protein content in the prepared starch, which reached up to 14.86 g/100 g wheat bran and 0.25%, respectively. The prepared starch contained lower contents of both protein and damaged starch and small starch granules with a volume fraction of 58.4%. Compared with wheat starch, this starch exhibited lower amylose content, pasting value and degree of crystallinity and higher swelling power.

Key words: wheat, bran, starch, neutrase

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